Die Glucose-Oxydasen aus Penicillium notatum (Notatin) und Aspergillus niger (Nigerin) I. Isolierung und einige molekulare eigenschaften

1965

Summary By chromatography on carboxymethyl- and diethylaminoethyl-Sephadex pure preparations of the glucose oxidases ( β - d -glucose: oxygen oxidoreductase, EC 1.1.3.4) notatin from Penicillium notatum and nigerin from Aspergillus niger could be obtained. Differences in the binding to the cation- and anion-exchangers indicate that these two glucose oxidases differ markedly with respect to the structures of the respective apoenzymes. A mixture of notatin and nigerin can easily be separated by chromatography. Moreover, there are also structural differences in the neighbourhood of the prosthetic flavin-adenine dinucleotide groups. The flavin-adenine dinucleotide spectrum of notatin is shifted…