0000000000147397
AUTHOR
Jane Webster
The DrosDel Collection
Abstract We describe a collection of P-element insertions that have considerable utility for generating custom chromosomal aberrations in Drosophila melanogaster. We have mobilized a pair of engineered P elements, p{RS3} and p{RS5}, to collect 3243 lines unambiguously mapped to the Drosophila genome sequence. The collection contains, on average, an element every 35 kb. We demonstrate the utility of the collection for generating custom chromosomal deletions that have their end points mapped, with base-pair resolution, to the genome sequence. The collection was generated in an isogenic strain, thus affording a uniform background for screens where sensitivity to genetic background is high. The…
How the AIS can Improve its Contributions to the UN’s Sustainability Development Goals: Towards A Framework for Scaling Collaborations and Evaluating Impact
In March, 2020, the coronavirus disease of 2019 (COVID-19) pandemic affected the information systems (IS) higher education community (along with the rest of the world) profoundly. Higher education institutes across the world had to quickly shift to online courses. In some cases, faculty had to transition their courses in only days. In response, the Communications of the Association for Information Systems launched a special issue on COVID-19, learning, pedagogy, and educational systems to provide a forum for IS faculty around the world to share effective practices and opinions regarding the long-term consequences of the COVID-19 pandemic on IS education. This paper serves as the editorial f…
The DrosDel Deletion Collection: A Drosophila Genomewide Chromosomal Deficiency Resource
AbstractWe describe a second-generation deficiency kit for Drosophila melanogaster composed of molecularly mapped deletions on an isogenic background, covering ∼77% of the Release 5.1 genome. Using a previously reported collection of FRT-bearing P-element insertions, we have generated 655 new deletions and verified a set of 209 deletion-bearing fly stocks. In addition to deletions, we demonstrate how the P elements may also be used to generate a set of custom inversions and duplications, particularly useful for balancing difficult regions of the genome carrying haplo-insufficient loci. We describe a simple computational resource that facilitates selection of appropriate elements for generat…