3D-QSAR study of ligands for a human olfactory receptor

Abstract Only about 350 olfactory receptors (OR) provide a basis for the remarkable ability of humans to recognise and discriminate a large number of odorants. A recent study reports the odorant repertoire of a human class II OR called OR1G1, including both agonists and antagonists. We used these affinity data to perform a 3D molecular modelling study of these ligands using Catalyst/HypoGen software (Catalyst v4.9.1, Accelrys Inc., San Diego, 2004) to propose alignment models for OR1G1 ligands. We obtained a triple-alignment model, which satisfactorily explained the experimental activities and was able both to predict the antagonist effects of some compounds and to identify new potent agoni…

Xenopus Laevis and Xenopus Tropicalis have different number of OBP genes : Xlaeobp and Xtroobp

International audience

TASTE RECEPTORS FOR GLUTAMATE IN HUMAN FUNGIFORM PAPILLAE

International audience

Characterization of an olfactory binding protein Xtro OBP in xenopus tropicalis in the aerial cavity

Tas1R1-Tas1R3 taste receptor variants in human fungiform papillae.

International audience; Monosodium glutamate as well as metabotropic and ionotropic glutamate receptor agonists have been reported to be perceived as umami by humans. In spite of the fact that Tas1R1-Tas1R3 has been shown to mediate most of the glutamate taste sensation in mice other candidate receptors have been put forward for which a clear role in detection is still lacking. This work was aimed at investigating the molecular determinants underlying umami taste detection in humans. First, we show evidence supporting expression of Tas1R1 and Tas1R3 but not mGluRs in the fungiform papillae of several individuals. Next, we report a number of naturally occurring l-glutamate taste receptor var…