0000000000154117

AUTHOR

Alfredo Quiñones-hinojosa

showing 11 related works from this author

Melatonin Targets Metabolism in Head and Neck Cancer Cells by Regulating Mitochondrial Structure and Function.

2021

This study was funded by grants from the Ministerio de Economia, Industria y Competitividad y por el Fondo de Desarrollo Regional FEDER, Spain nº SAF2013-49019, SAF2017-85903-P, and from the Consejería de Innovación, Ciencia y Empresa, Junta de Andalucía (P07- CTS- 03135, P10- CTS- 5784, and CTS- 101), Spain. J.F. and L.M. have FPU fellowships from the Ministerio de Educación Cultura y Deporte, Spain. C.R.S. was a schorlarship holder from the Plan Propio de Investigación of the University of Granada.

0301 basic medicine:Phenomena and Processes::Chemical Phenomena::Biochemical Phenomena::Biochemical Processes::Phosphorylation::Oxidative Phosphorylation [Medical Subject Headings]PhysiologyClinical BiochemistrymelatoninMitochondrionBiochemistryMelatonina:Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::Humans [Medical Subject Headings]0302 clinical medicine:Anatomy::Cells::Cells Cultured::Cell Line [Medical Subject Headings]head and neck cancer cells:Phenomena and Processes::Physiological Phenomena::Pharmacological Phenomena::Drug Resistance::Drug Resistance Neoplasm [Medical Subject Headings]MitophagyMitocondriasChemistryapoptosisglycolysisOXPHOSmitochondria030220 oncology & carcinogenesishormones hormone substitutes and hormone antagonistsmedicine.drug:Phenomena and Processes::Chemical Phenomena::Biochemical Phenomena::Biochemical Processes::Carbohydrate Metabolism::Glycolysis [Medical Subject Headings]Neoplasias de cabeza y cuello:Diseases::Neoplasms::Neoplasms by Site::Head and Neck Neoplasms [Medical Subject Headings]:Chemicals and Drugs::Inorganic Chemicals::Free Radicals::Reactive Oxygen Species [Medical Subject Headings]Mitofagiafree radicalsOxidative phosphorylationArticleMelatonin03 medical and health sciencesmedicine:Phenomena and Processes::Cell Physiological Phenomena::Cell Physiological Processes::Cell Growth Processes::Cell Proliferation [Medical Subject Headings]Molecular BiologyRadicales libresCell growth:Chemicals and Drugs::Amino Acids Peptides and Proteins::Proteins::DNA-Binding Proteins::Receptors Cytoplasmic and Nuclear::Receptors Melatonin [Medical Subject Headings]:Chemicals and Drugs::Chemical Actions and Uses::Pharmacologic Actions::Therapeutic Uses::Antineoplastic Agents [Medical Subject Headings]lcsh:RM1-950:Anatomy::Cells::Cellular Structures::Subcellular Fractions::Mitochondria [Medical Subject Headings]Cell Biologymedicine.diseaseHead and neck squamous-cell carcinoma:Phenomena and Processes::Cell Physiological Phenomena::Cell Physiological Processes::Cell Death::Apoptosis [Medical Subject Headings]Glucólisis030104 developmental biologylcsh:Therapeutics. PharmacologymitophagyApoptosisCancer cellCancer research:Chemicals and Drugs::Hormones Hormone Substitutes and Hormone Antagonists::Hormones::Melatonin [Medical Subject Headings]
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Subventricular zone localized irradiation affects the generation of proliferating neural precursor cells and the migration of neuroblasts

2012

Radiation therapy is a part of the standard treatment for brain tumor patients, often resulting in irreversible neuropsychological deficits. These deficits may be due to permanent damage to the neural stem cell (NSC) niche, damage to local neural progenitors, or neurotoxicity. Using a computed tomography-guided localized radiation technique, we studied the effects of radiation on NSC proliferation and neuroblast migration in the mouse brain. Localized irradiation of the subventricular zone (SVZ) eliminated the proliferating neural precursor cells and migrating neuroblasts. After irradiation, type B cells in the SVZ lacked the ability to generate migrating neuroblasts. Neuroblasts from the u…

Maleanimal structuresanimal diseasesSubventricular zoneCell CountBiologyArticleCerebral VentriclesMiceNeuroblastNeural Stem CellsCell MovementPrecursor cellNeuroblast migrationSpheroids CellularmedicineAnimalsreproductive and urinary physiologyCells CulturedCell ProliferationNeurogenesisCell migrationCell BiologyOlfactory BulbNeural stem cellCell biologyMice Inbred C57BLmedicine.anatomical_structurenervous systemAstrocytesImmunologyMolecular MedicineGanglion mother cellDevelopmental Biology
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Age-related changes in astrocytic and ependymal cells of the subventricular zone

2014

Neurogenesis persists in the adult subventricular zone (SVZ) of the mammalian brain. During aging, the SVZ neurogenic capacity undergoes a progressive decline, which is attributed to a decrease in the population of neural stem cells (NSCs). However, the behavior of the NSCs that remain in the aged brain is not fully understood. Here we performed a comparative ultrastructural study of the SVZ niche of 2-month-old and 24-month-old male C57BL/6 mice, focusing on the NSC population. Using thymidine-labeling, we showed that residual NSCs in the aged SVZ divide less frequently than those in young mice. We also provided evidence that ependymal cells are not newly generated during senescence, as ot…

Senescenceeducation.field_of_studyEpendymal CellCellular differentiationPopulationNeurogenesisSubventricular zoneBiologyNeural stem cellCellular and Molecular Neurosciencemedicine.anatomical_structurenervous systemNeurologymedicineeducationEpendymaNeuroscienceGlia
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Intra-operatively obtained human tissue: Protocols and techniques for the study of neural stem cells

2009

The discoveries of neural (NSCs) and brain tumor stem cells (BTSCs) in the adult human brain and in brain tumors, respectively, have led to a new era in neuroscience research. These cells represent novel approaches to studying normal phenomena such as memory and learning, as well as pathological conditions such as Parkinson's disease, stroke, and brain tumors. This new paradigm stresses the importance of understanding how these cells behave in vitro and in vivo. It also stresses the need to use human-derived tissue to study human disease because animal models may not necessarily accurately replicate the processes that occur in humans. An important, but often underused, source of human tissu…

BiopsyBrain tumorCell Culture TechniquesNerve Tissue ProteinsBiologyArticleIntraoperative PeriodIn vivoNeurosphereSpheroids CellularmedicineElectron microscopyHumansProcess (anatomy)NeuronsNeural stem cellsBrain NeoplasmsGeneral NeuroscienceStem CellsBrain tumor stem cellsHuman brainmedicine.diseaseImmunohistochemistryNeural stem cellCulture MediaMicroscopy Electronmedicine.anatomical_structureCell cultureAstrocytesNeoplastic Stem CellsTissue and Organ HarvestingNeurospheresStem cellNeuroscienceBiomarkersImmunocytochemistry
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The human brain subventricular zone: stem cells in this niche and its organization.

2007

The human brain harbors stem cells in the subventricular zone (SVZ). The authors have collected postmortem and intraoperative tissue from adult human patients and found that it contains a unique ribbon of astrocytes that proliferate in vivo and can function as neural stem cells in vitro. Furthermore, they have conducted an anatomic, cytoarchitectural, and ultrastructural study in complete postmortem brains to define the precise organization of the lateral walls of the human lateral ventricles. With immunohistochemistry, the authors mapped a proliferative glial fibrillary acidic protein (GFAP)--positive ribbon of astrocytic cells in the human SVZ. In this article, the authors report on four …

NeuronsGlial fibrillary acidic proteinbiologybusiness.industryHippocampusSubventricular zoneGeneral MedicineHuman brainNeural stem cellCell biologyCerebral VentriclesLateral ventriclesAdult Stem Cellsmedicine.anatomical_structurenervous systemAstrocytesNeural Pathwaysmedicinebiology.proteinImmunohistochemistryHumansSurgeryNeurology (clinical)Stem cellbusinessNeurosurgery clinics of North America
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PDGFRα-Positive B Cells Are Neural Stem Cells in the Adult SVZ that Form Glioma-like Growths in Response to Increased PDGF Signaling

2006

Neurons and oligodendrocytes are produced in the adult brain subventricular zone (SVZ) from neural stem cells (B cells), which express GFAP and have morphological properties of astrocytes. We report here on the identification B cells expressing the PDGFRalpha in the adult SVZ. Specifically labeled PDGFRalpha expressing B cells in vivo generate neurons and oligodendrocytes. Conditional ablation of PDGFRalpha in a subpopulation of postnatal stem cells showed that this receptor is required for oligodendrogenesis, but not neurogenesis. Infusion of PDGF alone was sufficient to arrest neuroblast production and induce SVZ B cell proliferation contributing to the generation of large hyperplasias wi…

Receptor Platelet-Derived Growth Factor alphaAdolescentNeuroscience(all)Subventricular zoneMice TransgenicDEVBIOBiologyMOLNEUROMiceNeuroblastLateral VentriclesmedicineAnimalsHumansCell ProliferationAged 80 and overNeuronsPlatelet-Derived Growth FactorStem CellsGeneral NeuroscienceNeurogenesisGliomaMiddle AgedSTEMCELLOligodendrocyteNeural stem cellCell biologymedicine.anatomical_structurenervous systemNeuronStem cellNeuroscienceSignal TransductionAdult stem cellNeuron
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Cellular composition and cytoarchitecture of the adult human subventricular zone: A niche of neural stem cells

2005

The lateral wall of the lateral ventricle in the human brain contains neural stem cells throughout adult life. We conducted a cytoarchitectural and ultrastructural study in complete postmortem brains (n = 7) and in postmortem (n = 42) and intraoperative tissue (n = 43) samples of the lateral walls of the human lateral ventricles. With varying thickness and cell densities, four layers were observed throughout the lateral ventricular wall: a monolayer of ependymal cells (Layer I), a hypocellular gap (Layer II), a ribbon of cells (Layer III) composed of astrocytes, and a transitional zone (Layer IV) into the brain parenchyma. Unlike rodents and nonhuman primates, adult human glial fibrillary a…

AdultEpendymal CellAdolescentSubventricular zoneLateral ventriclesProsencephalonEpendymaLateral VentriclesmedicineHumansChildNeuronsGlial fibrillary acidic proteinbiologyStem CellsGeneral NeuroscienceNeurogenesisCell DifferentiationAnatomyMiddle AgedImmunohistochemistryNeural stem cellCell biologymedicine.anatomical_structurenervous systemAstrocytesbiology.proteinStem cellEpendymaThe Journal of Comparative Neurology
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Unique astrocyte ribbon in adult human brain contains neural stem cells but lacks chain migration

2003

The subventricular zone (SVZ) is a principal source of adult neural stem cells in the rodent brain, generating thousands of olfactory bulb neurons every day. If the adult human brain contains a comparable germinal region, this could have considerable implications for future neuroregenerative therapy. Stem cells have been isolated from the human brain, but the identity, organization and function of adult neural stem cells in the human SVZ are unknown. Here we describe a ribbon of SVZ astrocytes lining the lateral ventricles of the adult human brain that proliferate in vivo and behave as multipotent progenitor cells in vitro. This astrocytic ribbon has not been observed in other vertebrates s…

AdultBiopsyanimal diseasesSubventricular zoneBiologyCell MovementNeurospheremedicineHumansCells CulturedNeuronsMultidisciplinaryMultipotent Stem CellsNeurogenesisBrainCell DifferentiationAnatomyOlfactory BulbNeural stem cellNeuroepithelial cellNeuropoiesismedicine.anatomical_structurenervous systemAstrocytesAutopsyStem cellNeuroscienceCell DivisionAdult stem cellNature
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The generation of oligodendroglial cells is preserved in the rostral migratory stream during aging

2013

The subventricular zone (SVZ) is the largest source of newly generated cells in the adult mammalian brain. SVZ-derived neuroblasts migrate via the rostral migratory stream (RMS) to the olfactory bulb (OB), where they differentiate into mature neurons. Additionally, a small proportion of SVZ-derived cells contribute to the generation of myelinating oligodendrocytes. The production of new cells in the SVZ decreases during aging, affecting the incorporation of new neurons into the OB. However, the age-related changes that occur across the RMS are not fully understood. In this study we evaluate how aging affects the cellular organization of migrating neuroblast chains, the proliferation, and th…

SenescenceAgingneuroblast migrationRostral migratory streamSubventricular zoneCèl·lulesNeurogenesisRostral migratory streamSubventricular zoneNeuronesBiologylcsh:RC321-57103 medical and health sciencesCellular and Molecular NeuroscienceNeurologia0302 clinical medicineNeuroblastoligodendrogenesisNeuroblast migrationmedicineOriginal Research Articlelcsh:Neurosciences. Biological psychiatry. Neuropsychiatry030304 developmental biology0303 health sciencesNeurogenesisOlfactory BulbOligodendrocyteOlfactory bulbmedicine.anatomical_structurenervous systemNeuroscience030217 neurology & neurosurgeryNeuroscienceFrontiers in Cellular Neuroscience
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Preservation of glial cytoarchitecture from ex vivo human tumor and non-tumor cerebral cortical explants: A human model to study neurological diseases

2007

For the human brain, in vitro models that accurately represent what occurs in vivo are lacking. Organotypic models may be the closest parallel to human brain tissue outside of a live patient. However, this model has been limited primarily to rodent-derived tissue. We present an organotypic model to maintain intraoperatively collected human tumor and non-tumor explants ex vivo for a prolonged period of time (similar to 11 days) without any significant changes to the tissue cytoarchitecture as evidenced through immunohistochemistry and electron microscopy analyses. The ability to establish and reliably predict the cytoarchitectural changes that occur with time in an organotypic model of tumor…

Pathologymedicine.medical_specialtyIndolesTime FactorsbrainMatrix (biology)BiologyModels BiologicalStatistics NonparametricArticleOrgan Culture TechniquesMicroscopy Electron TransmissionIn vivoGlial Fibrillary Acidic ProteinmedicineHumanshumanorganotypicCerebral Cortexelectron microscopyBrain NeoplasmsGeneral NeuroscienceexplantReproducibility of ResultsCell migrationHuman brainMiddle AgedImmunohistochemistrymedicine.anatomical_structureCytoarchitectureImmunohistochemistryFemaleTissue PreservationNervous System DiseasesNeurogliaEx vivoExplant culture
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Age-Related Changes in Astrocytic and Ependymal Cells of the Subventricular Zone

2014

Neurogenesis persists in the adult subventricular zone (SVZ) of the mammalian brain. During aging, the SVZ neurogenic capacity undergoes a progressive decline, which is attributed to a decrease in the population of neural stem cells (NSCs). However, the behavior of the NSCs that remain in the aged brain is not fully understood. Here we performed a comparative ultrastructural study of the SVZ niche of 2-month-old and 24-month-old male C57BL/6 mice, focusing on the NSC population. Using thymidine-labeling, we showed that residual NSCs in the aged SVZ divide less frequently than those in young mice. We also provided evidence that ependymal cells are not newly generated during senescence, as ot…

nervous systemagingastrocytesependymal cellssubventricular zoneultrastructureneural stem cells
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