0000000000154298

AUTHOR

Peter Vandamme

showing 3 related works from this author

Development of a database for the rapid and accurate routine identification of Achromobacter species by matrix-assisted laser desorption/ionization-t…

2019

International audience; Objectives: Achromobacter spp. are emerging pathogens in respiratory samples from cystic fibrosis patients. The current reference methods (nrdA-sequencing or multilocus sequence typing) can identify 18 species which are often misidentified by conventional techniques as A. xylosoxidans. A few studies have suggested that matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF/MS) provides accurate identification of the genus but not of species. The aims of this study were (a) to generate a database for MALDI-TOF/MS Bruker including the 18 species, (b) to evaluate the suitability of the database for routine laboratory identification, and …

0301 basic medicineMicrobiology (medical)MALDI-TOFAchromobacter speciesAchromobacterDatabases FactualRibonucleoside Diphosphate Reductase030106 microbiologyspecies identificationMatrix assisted laser desorption ionization time of flightAchromobacterBiologyMass spectrometrycomputer.software_genre03 medical and health sciences0302 clinical medicineHumans030212 general & internal medicineRespiratory samplesmass spectrometryDatabaseDiagnostic Tests RoutineGeneral Medicinebiology.organism_classification[SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/BacteriologynrdAIdentification (information)Matrix-assisted laser desorption/ionizationInfectious DiseasesSpectrometry Mass Matrix-Assisted Laser Desorption-IonizationMultilocus sequence typing[SDV.MP.BAC] Life Sciences [q-bio]/Microbiology and Parasitology/BacteriologyGram-Negative Bacterial InfectionscomputerSoftwareClinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases
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Polyphasic taxonomic characterization of Lactobacillus rossiae isolates from Belgian and Italian sourdoughs reveals intraspecific heterogeneity.

2009

Abstract (GTG) 5 -PCR fingerprinting and pheS sequence analysis of 18 Lactobacillus rossiae isolates, mainly originating from Belgian and Italian artisan sourdoughs, revealed intraspecies grouping as evidenced by the delineation of three and two subgroups, respectively. On the other hand, 16S rRNA and rpoA gene sequence analysis and DNA–DNA hybridizations supported the accommodation of all isolates in a single species. No correlation between genetic and phenotypic heterogeneity was observed. Collectively, these data do not warrant taxonomic division of L. rossiae . On the other hand, the considerable differences in intraspecies sequence variation of L. rossiae isolates displayed by the pheS…

DNA BacterialRpoaSequence analysisMolecular Sequence DataBiologyApplied Microbiology and BiotechnologyMicrobiologyGenomeDNA Ribosomallaw.inventionBelgiumSpecies SpecificitylawRNA Ribosomal 16SGene(GTG)5-PCREcology Evolution Behavior and SystematicsPolymerase chain reactionGeneticsGenetic heterogeneityNucleic Acid HybridizationLactobacillus rossiae tassonomia polifasicaBreadDNA-Directed RNA PolymerasesSequence Analysis DNA16S ribosomal RNALactobacillus rossiaeDNA FingerprintingHousekeeping geneBacterial Typing TechniquesLactobacillusPhenotypeItalyGenetic markerPhesPhenylalanine-tRNA LigasePolyphasic taxonomySystematic and applied microbiology
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gcType : a high-quality type strain genome database for microbial phylogenetic and functional research

2020

Abstract Taxonomic and functional research of microorganisms has increasingly relied upon genome-based data and methods. As the depository of the Global Catalogue of Microorganisms (GCM) 10K prokaryotic type strain sequencing project, Global Catalogue of Type Strain (gcType) has published 1049 type strain genomes sequenced by the GCM 10K project which are preserved in global culture collections with a valid published status. Additionally, the information provided through gcType includes >12 000 publicly available type strain genome sequences from GenBank incorporated using quality control criteria and standard data annotation pipelines to form a high-quality reference database. This …

Data AnalysisBACTERIALAcademicSubjects/SCI000100206 medical engineering02 engineering and technologyComputational biologyBiologyGenome03 medical and health sciencesMULTIPLE SEQUENCE ALIGNMENTPhylogeneticsRNA Ribosomal 16SDatabases GeneticGeneticsPROGRAMDatabase IssueALGORITHMPhylogeny030304 developmental biology0303 health sciencesGenomeMultiple sequence alignmentBase SequencePhylogenetic treeResearchGenome databaseBiology and Life SciencesGCM transcription factorsProkaryotic CellsGenBankReference database020602 bioinformatics
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