0000000000154707
AUTHOR
Jana Schwarz-linek
showing 2 related works from this author
Polymer-induced phase separation in suspensions of bacteria
2010
We study phase separation in suspensions of two unrelated species of rod-like bacteria, Escherichia coli and Sinorhizobium meliloti, induced by the addition of two different anionic polyelectrolytes, sodium polystyrene sulfonate or succinoglycan, the former being synthetic and the latter of natural origin. Comparison with the known behaviour of synthetic colloid-polymer mixtures and with simulations show that "depletion" (or, equivalently, "macromolecular crowding") is the dominant mechanism: exclusion of the non-adsorbing polymer from the region between two neighbouring bacteria creates an unbalanced osmotic force pushing them together. The implications of our results for understanding phe…
Polymer-induced phase separation in Escherichia coli suspensions
2010
We studied aggregation and phase separation in suspensions of de-flagellated Escherichia coli (AB1157) in phosphate buffer induced by the anionic polyelectrolyte sodium polystyrene sulfonate. We also performed Monte Carlo simulations of this system based on the Asakura–Oosawa model of colloid–polymer mixtures. The results of these simulations, as well as comparison with previous work on synthetic colloid–polymer mixtures, demonstrate that the role of the polymer is to cause a depletion attraction between the E. coli cells. The implication of these results for understanding the role of (predominantly anionic) extracellular polymeric substances (EPS) secreted by bacteria in various natural ph…