0000000000156982

AUTHOR

Elke Duchardt-ferner

showing 3 related works from this author

Secondary structure determination of conserved SARS-CoV-2 RNA elements by NMR spectroscopy

2020

AbstractThe current pandemic situation caused by the Betacoronavirus SARS-CoV-2 (SCoV2) highlights the need for coordinated research to combat COVID-19. A particularly important aspect is the development of medication. In addition to viral proteins, structured RNA elements represent a potent alternative as drug targets. The search for drugs that target RNA requires their high-resolution structural characterization. Using nuclear magnetic resonance (NMR) spectroscopy, a worldwide consortium of NMR researchers aims to characterize potential RNA drug targets of SCoV2. Here, we report the characterization of 15 conserved RNA elements located at the 5′ end, the ribosomal frameshift segment and t…

Untranslated region0303 health sciencesAcademicSubjects/SCI00010Base pairNAR Breakthrough ArticleRNANuclear magnetic resonance spectroscopyComputational biologyBiology010402 general chemistry01 natural sciencesGenomeFootprintingRibosomal frameshift0104 chemical sciences03 medical and health sciencesGeneticsProtein secondary structure030304 developmental biologyNucleic Acids Research
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Structural Basis of TRPV4 N Terminus Interaction with Syndapin/PACSIN1-3 and PIP2

2018

Summary Transient receptor potential (TRP) channels are polymodally regulated ion channels. TRPV4 (vanilloid 4) is sensitized by PIP2 and desensitized by Syndapin3/PACSIN3, which bind to the structurally uncharacterized TRPV4 N terminus. We determined the nuclear magnetic resonance structure of the Syndapin3/PACSIN3 SH3 domain in complex with the TRPV4 N-terminal proline-rich region (PRR), which binds as a class I polyproline II (PPII) helix. This PPII conformation is broken by a conserved proline in a cis conformation. Beyond the PPII, we find that the proximal TRPV4 N terminus is unstructured, a feature conserved across species thus explaining the difficulties in resolving it in previous …

0301 basic medicineChemistryAffinitiesSH3 domainN-terminus03 medical and health sciencesTransient receptor potential channel030104 developmental biologyStructural biologyStructural BiologyHelixBiophysicslipids (amino acids peptides and proteins)Molecular BiologyIon channelPolyproline helixStructure
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Correction to ‘Secondary structure determination of conserved SARS-CoV-2 RNA elements by NMR spectroscopy’

2021

The current pandemic situation caused by the Betacoronavirus SARS-CoV-2 (SCoV2) highlights the need for coordinated research to combat COVID-19. A particularly important aspect is the development of medication. In addition to viral proteins, structured RNA elements represent a potent alternative as drug targets. The search for drugs that target RNA requires their high-resolution structural characterization. Using nuclear magnetic resonance (NMR) spectroscopy, a worldwide consortium of NMR researchers aims to characterize potential RNA drug targets of SCoV2. Here, we report the characterization of 15 conserved RNA elements located at the 5' end, the ribosomal frameshift segment and the 3'-un…

Models Molecular2019-20 coronavirus outbreakMagnetic Resonance SpectroscopyCoronavirus disease 2019 (COVID-19)AcademicSubjects/SCI00010Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)Genome ViralBiology03 medical and health sciencesGeneticsHumans3' Untranslated RegionsPandemicsProtein secondary structure030304 developmental biology0303 health sciencesBase SequenceSARS-CoV-2030302 biochemistry & molecular biologyCOVID-19Frameshifting RibosomalRNANuclear magnetic resonance spectroscopyVirologyNucleic Acid ConformationRNA ViralCorrigendumNucleic Acids Research
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