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RESEARCH PRODUCT
Correction to ‘Secondary structure determination of conserved SARS-CoV-2 RNA elements by NMR spectroscopy’
Anna WackerJulia E. WeigandSabine R AkabayovNadide AltincekicJasleen Kaur BainsElnaz BanijamaliOliver BinasJesus Castillo-martinezErhan CetinerBetül CeylanLiang-yuan ChiuJesse Davila-calderonKarthikeyan DhamotharanElke Duchardt-fernerJan FernerLucio FrydmanBoris FürtigJosé GallegoJ Tassilo GrünCarolin HackerChristina HaddadMartin HähnkeMartin HengesbachFabian HillerKatharina F HohmannDaniel HymonVanessa De JesusHenry JonkerHeiko KellerBozana KnezicTom LandgrafFrank LöhrLe LuoKlara R MertinkusChristina MuhsMihajlo NovakovicAndreas OxenfarthMartina Palomino-schätzleinKatja PetzoldStephen A PeterDennis J PyperNusrat S QureshiMagdalena RiadChristian RichterKrishna SaxenaTatjana SchamberTali ScherfJudith SchlagnitweitAndreas SchlundtRobbin SchniedersHarald SchwalbeAlvaro Simba-lahuasiSridhar SreeramuluElke StirnalAlexey SudakovJan-niklas TantsBlanton S TolbertJennifer VögeleLena WeißJulia Wirmer-bartoschekMaria A Wirtz MartinJens WöhnertHeidi Zetzschesubject
Models Molecular2019-20 coronavirus outbreakMagnetic Resonance SpectroscopyCoronavirus disease 2019 (COVID-19)AcademicSubjects/SCI00010Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)Genome ViralBiology03 medical and health sciencesGeneticsHumans3' Untranslated RegionsPandemicsProtein secondary structure030304 developmental biology0303 health sciencesBase SequenceSARS-CoV-2030302 biochemistry & molecular biologyCOVID-19Frameshifting RibosomalRNANuclear magnetic resonance spectroscopyVirologyNucleic Acid ConformationRNA ViralCorrigendumdescription
The current pandemic situation caused by the Betacoronavirus SARS-CoV-2 (SCoV2) highlights the need for coordinated research to combat COVID-19. A particularly important aspect is the development of medication. In addition to viral proteins, structured RNA elements represent a potent alternative as drug targets. The search for drugs that target RNA requires their high-resolution structural characterization. Using nuclear magnetic resonance (NMR) spectroscopy, a worldwide consortium of NMR researchers aims to characterize potential RNA drug targets of SCoV2. Here, we report the characterization of 15 conserved RNA elements located at the 5' end, the ribosomal frameshift segment and the 3'-untranslated region (3'-UTR) of the SCoV2 genome, their large-scale production and NMR-based secondary structure determination. The NMR data are corroborated with secondary structure probing by DMS footprinting experiments. The close agreement of NMR secondary structure determination of isolated RNA elements with DMS footprinting and NMR performed on larger RNA regions shows that the secondary structure elements fold independently. The NMR data reported here provide the basis for NMR investigations of RNA function, RNA interactions with viral and host proteins and screening campaigns to identify potential RNA binders for pharmaceutical intervention.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2021-06-23 | Nucleic Acids Research |