0000000000157034

AUTHOR

A. Buling

showing 2 related works from this author

Hemoprotozoa of domestic animals in France: Prevalence and molecular characterization

2008

Very limited information is available on epizootiology of haematozoan infections in French domestic animals. In an attempt to address this issue, prevalence of piroplasmida was studied in carnivores and ruminants, whereas prevalence of Hepatozoon spp. was only investigated in carnivores. In total, 383 animals were included in the survey (namely 116 cats, 108 dogs, 91 sheep and 68 cows). Parasite diagnosis was carried out using molecular methods such as PCR and sequencing of the 18S rRNA gene. In addition, ruminant samples were analyzed with the reverse line blotting technique (RLB). Results of RLB and PCR plus sequencing were in total agreement. In carnivores, haematozoan prevalence was clo…

Veterinary medicinePrevalenceBabesiaPolymerase Chain ReactionDogsBabesiosisTheileriaparasitic diseasesTheileriaPrevalenceRNA Ribosomal 18SAnimalsPiroplasmidaSheepGeneral VeterinarybiologySequence Analysis RNAData CollectionEpizootiology Hepatozoon canis Piroplasmida PCRGeneral MedicineCytauxzoonosisbiology.organism_classificationTheileriasisCytauxzoonCanisAnimals DomesticBabesiaCatsBabesia canisCattleParasitologyFranceVeterinary Parasitology
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Development of fluorogenic probe-based PCR assays for the detection and quantification of bovine piroplasmids.

2009

This paper reports two new quantitative PCR (qPCR) assays, developed in an attempt to improve the detection of bovine piroplasmids. The first of these techniques is a duplex TaqMan assay for the simultaneous diagnosis of Babesia bovis and B. bigemina. This technique is ideal for use in South America where bovids harbour no theilerids. The second technique, which is suitable for the diagnosis of both babesiosis and theileriosis worldwide, involves fluorescence resonance energy transfer (FRET) probes. In FRET assays, Babesia bovis, B. divergens, Babesia sp. (B. major or B. bigemina), Theileria annae and Theileria sp. were all identifiable based on the melting temperatures of their amplified f…

BabesiaPolymerase Chain ReactionSensitivity and Specificitylaw.inventionlawBabesiosisTheileriaTheileriaFluorescence Resonance Energy TransferTaqManmedicineAnimalsHorsesBabesia bigeminaPolymerase chain reactionGeneral VeterinarybiologyReproducibility of ResultsBabesia bovisBabesiosisGeneral MedicineDNA Protozoanbiology.organism_classificationmedicine.diseaseVirologyMolecular biologyBabesiaTheileria Quantitative PCR Molecular diagnostic TaqMan probes FRET probesTheileriasisReal-time polymerase chain reactionBabesiaCattleHorse DiseasesParasitology
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