0000000000165279
AUTHOR
Marc Garcia-garcera
A New Method for Extracting Skin Microbes Allows Metagenomic Analysis of Whole-Deep Skin
In the last decade, an extensive effort has been made to characterize the human microbiota, due to its clinical and economic interests. However, a metagenomic approach to the skin microbiota is hampered by the high proportion of host DNA that is recovered. In contrast with the burgeoning field of gut metagenomics, skin metagenomics has been hindered by the absence of an efficient method to avoid sequencing the host DNA. We present here a method for recovering microbial DNA from skin samples, based on a combination of molecular techniques. We have applied this method to mouse skin, and have validated it by standard, quantitative PCR and amplicon sequencing of 16S rRNA. The taxonomic diversit…
No Major Host Genetic Risk Factor Contributed to A(H1N1)2009 Influenza Severity
Garcia-Etxebarria, Koldo et al.
Staphylococcus prevails in the skin microbiota of long-term immunodeficient mice
Summary Host-commensal relationships in the skin are a complex system governed by variables related to the host, the bacteria and the environment. A disruption of this system may lead to new steady states, which, in turn, may lead to disease. We have studied one such disruption by characterizing the skin micro- biota in healthy and immunodepressed (ID) mice. A detailed anatomopathological study failed to reveal any difference between the skin of healthy and ID mice. We sequenced the 16S rDNA V1-V2 gene region to saturation in 10 healthy and 10 ID 8 week- old mice, and found than all of the healthy and two of the ID mice had bacterial communities that were similar in composition to that of h…
Direct squencing from the minimal number of DNA molecules needed to fill a 454 picotiterplate
Notice of Republication: This article was republished on June 17, 2014, to correct an error in the title. The publisher apologizes for the error. In addition, a typographical error was corrected in the Abstract. Please download this article again to view the correct version. The originally published, uncorrected article and the republished, corrected article are provided here for reference.