0000000000172073
AUTHOR
Richard A. Holley
Combination of phenolic acids and essential oils against Listeria monocytogenes
Abstract Listeria monocytogenes is a psychrotrophic pathogen widely distributed in meat processing plants. Listeriosis presents very low morbidity, but very high mortality rates. Several outbreaks involving this bacterium have been reported due to the consumption of refrigerated meat and dairy products. The objective of the present study was to evaluate the in vitro antimicrobial activity of 10 phenolic acids (PAs) and 4 essential oil compounds (EOCs) as natural alternatives to control L. monocytogenes growth. The minimum inhibitory concentration (MIC) of all natural compounds was determined at pH 5, 6 and 7. The 4 most potent PAs (ρ-hydroxybenzoic acid, ferulic acid, o-coumaric acid and sy…
Microbial and chemical origins of the bactericidal activity of thermally treated yellow mustard powder toward Escherichia coli O157:H7 during dry sausage ripening
Abstract Work examines the origin of bactericidal activity in mustard flour and explores the relative contribution from starter cultures, E. coli O157:H7 itself and other sources. Bacteria can degrade naturally occurring glucosinolates in mustard and form isothiocyanates with antimicrobial activity. In the present work, 24 starter cultures (mostly from commercial mixtures) were screened for their capacity to decompose the glucosinolate, sinalbin. The most active pair, Pediococcus pentosaceus UM 121P and Staphylococcus carnosus UM 123M, were used together for the production of dry fermented sausage contaminated with E. coli O157:H7 (~ 6.5 log CFU/g). They were compared to industrial starters…