0000000000173020
AUTHOR
Elisabetta Panza
Lauroside B, a Megastigmane Glycoside from Laurus Nobilis (Bay Laurel) Leaves, Induces Apoptosis in Human Melanoma Cell Lines by Inhibiting NF-κB Activation
Malignant melanoma is a highly aggressive tumor that frequently resists chemotherapy, so the search for new agents for its treatment is of great importance. In the present study, the antiproliferative propensity against human melanoma cell lines of lauroside B (1), a megastigmane glycoside isolated from Laurus nobilis (bay laurel) leaves, was investigated. This compound suppressed the proliferation of three human melanoma cell lines, namely, A375, WM115, and SK-Mel-28. The 1-induced inhibition of human melanoma cell proliferation was due to the induction of apoptosis, as demonstrated by FACS analysis with annexin V/PI staining and confirmed by activation of caspase-3 and by the cleavage of …
Terpioside B, a difucosyl GSL from the marine sponge Terpios sp. is a potent inhibitor of NO release.
Terpioside B (2a), a unique glycolipid containing two fucose residues in the furanose form in its pentasaccharide chain, was isolated from the marine sponge Terpios sp. Its complete stereostructure was solved by interpretation of mass spectrometric and NMR data along with CD and GG-MS analyses of its degradation products. Terpioside B is a potent inhibitor against LPS-induced NO release, and is considerably more active than simpler glycosphingolipids such as terpioside A and monoglucosylceramide.
Cactus pear fruit extract exerts anti-inflammatory effects in carrageenin-induced rat pleurisy
Nutritional research has recently shifted from alleviating nutrient deficiencies to chronic disease prevention. In this study activity of cactus pear fruit extract (CPFE) from Opuntia ficus-indica (L.) Mill. has been investigated in carrageenin-induced pleurisy, a rat model of acute inflammation. In our experimental design rat pleurisy was achieved by the injection of 0.2 ml of λ-carrageenin in the pleural cavity. At selected time points, rats were sacrificed; cells recruited in pleura were counted and exudates collected to analyse inflammatory parameters such as NO, PGE2, IL-1β, TNF-α. CPFE (in the range between 5 and 20 g fresh fruit equivalent/kg), orally given 30 min before the injectio…
Indicaxanthin from cactus pear fruit exerts anti-inflammatory effects in carrageenin-induced rat pleurisy.
Nutritional research has shifted recently from alleviating nutrient deficiencies to chronic disease prevention. We investigated the activity of indicaxanthin, a bioavailable phytochemical of the betalain class from the edible fruit of Opuntia ficus-indica (L. Miller) in a rat model of acute inflammation. Rat pleurisy was achieved by injection of 0.2 mL of l-carrageenin in the pleural cavity, and rats were killed 4, 24, and 48 h later; exudates were collected to analyze inflammatory parameters, such as nitric oxide (NO), prostaglandin E2 (PGE2), interleukin-1b (IL-1b), and tumor necrosis factor-a (TNF-a); cells recruited in pleura were analyzed for cyclooxygenase-2 (COX-2), inducible nitric …