0000000000174298
AUTHOR
N. Tusa
Response of cybrids and a somatic hybrid of lemon to Phoma tracheiphila infections
The reaction of lemon hybrids obtained by symmetrical and asymmetrical protoplast fusion, toward “mal secco” infection caused by Phoma tracheiphila (Petri) Kanc. et Ghik. was examined. Resistance was tested in `Valencia' sweet orange [Citrus sinensis (L.) Osbeck] and `Femminello' lemon [C. limon (L.) Burm. f.] somatic hybrid and cybrids, by stem and leaf inoculation tests and by analysis of propagule number of P. tracheiphila in the xylem of stem-inoculated plants. In general, the somatic hybrid and the cybrids showed an intermediate degree of resistance, with slight differences in disease symptoms, in comparison with resistant `Monachello' lemon and susceptible `Femminello' lemon, used as …
Citrus somatic hybrid leaf essential oil
The essential oil from the leaves of a Citrus interspecific somatic hybrid obtained by symmetric protoplast fusion between ‘Milam’ lemon [purported sexual hybrid of C. Jambhiri Lush (×) C. sinensis] and ‘Femminello’ lemon (C. limon L. Burm f.) has been characterized by steam distillation GC–MS analysis and its composition compared with that of the corresponding oils obtained from the parent plants. The amount (%) of the different classes of substances present in the oils show, in the new hybrid, an overall lowering of the hydrocarbon fraction and an increase of the oxygenated components. The somatic hybrid shows a close similarity in the amounts of terpenes, sesquiterpenes and alcohols with…
Characterization of five sour orange clones through molecular markers and leaf essential oils analysis.
Five clones of sour orange (Citrus aurantium L.) showing significant morphological differences were selected from our germplasm collection and characterized both by genetic and leaf volatiles analysis. The genetic studies were undertaken by the use of molecular markers developed by PCR-based techniques (ISSR and RAPD), while the leaf essential oil patterns were obtained by chromatographic and mass spectrometric determination. Data obtained suggest that reasonably similar information can be achieved from the two techniques, supporting each other in characterization studies.
Citrus cybrid leaf essential oil
The essential oil from the leaves of a Citrus cybrid plant, obtained by somatic hybridization between ‘Valencia’ sweet orange (Citrus sinensis L. Osbeck), embryogenic parent, and ‘Femminello’ lemon (C. limon L. Burm f.), leaf parent, has been studied by steam distillation and GC–MS analysis and compared with the correspondent oils from the parent plants. Although the overall composition of the cybrid essential oil appears much closer to that of ‘Femminello’, the relatively higher percentage of β-caryophyllene, as well as the qualitative and quantitative modifications of the esteric fraction, are characteristic of the new hybrid. Since cybrid cells possess the nuclear genome of the mesophyll…