0000000000177038
AUTHOR
Guillaume Lafforgue
Resistance to virus infection mediated by artificial microRNAs: estimating the likelilhood of escape mutants
Las plantas sufren las infecciones causadas por muchos patógenos como hongos, bacterias, virus y nematodos. Las infecciones virales son persistentes y frecuentemente no se curan por el sistema inmune como en los animales. Existe una alta gama de virus de plantas, alrededor de 450 especies bien caracterizadas, que cusan una alta gama de enfermedades en plantas. Enfrentando esta situación, las planas no son meros sujetos pasivos, sino que han desarrollado mecanismos defensivos elaborados y efectivos para prevenir, o limitar, los daños causados a las infecciones virales. Entre estos mecanismos de defensa, las plantas tienen genes que confieren resistencia a virios patógenos, incluyendo los vir…
Ultradeep Sequencing Analysis of Population Dynamics of Virus Escape Mutants in RNAi-Mediated Resistant Plants
Plant artificial micro-RNAs (amiRs) have been engineered to target viral genomes and induce their degradation. However, the exceptional evolutionary plasticity of RNA viruses threatens the durability of the resistance conferred by these amiRs. It has recently been shown that viral populations not experiencing strong selective pressure from an antiviral amiR may already contain enough genetic variability in the target sequence to escape plant resistance in an almost deterministic manner. Furthermore, it has also been shown that viral populations exposed to subinhibitory concentrations of the antiviral amiR speed up this process. In this article, we have characterized the molecular evolutiona…