0000000000179545
AUTHOR
And Andreas Janshoff
Scrutiny of annexin A1 mediated membrane-membrane interaction by means of a thickness shear mode resonator and computer simulations.
The dissipational quartz crystal microbalance (D-QCM) technology was applied to monitor the adsorption of vesicles to membrane-bound annexin A1 by simultaneously reading out the shifts in resonance frequency and dissipation. Solid-supported membranes (SSMs) composed of a chemisorbed octanethiol monolayer and a physisorbed 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine/1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoserine monolayer were immobilized on the gold electrode of a 5 MHz quartz plate. Adsorption and desorption of annexin A1 to the SSM was followed by means of the QCM technique. After nonbound annexin A1 was removed from solution, the second membrane binding was monitored by the D-QCM t…
Control of CaCO3 Crystallization by Demixing of Monolayers
In this paper we describe how to template a demixed monolayer into a spatially patterned inorganic replica. For this purpose a new amphiphilic monomer was synthesized which can be polymerized both in solution and in the monolayer of a Langmuir-Blodgett (LB) trough. Since it inhibits the crystallization of CaCO3, it can be used--in combination with stearic acid (nucleation-promotor)--to control CaCO3 crystals formed under the monolayer. Investigations of the two-component monolayer (Langmuir isotherms and AFM measurements of transferred films) show--in the biphasic region--demixing in solid analogue stearic acid domains and the liquid analogue phase of the monomer. Crystallization of CaCO3 s…