0000000000180345
AUTHOR
M. Abirached-darmency
Phylogenetic and genomic relationships in Setaria italica and its close relatives based on the molecular diversity and chromosomal organization of 5S and 18S-5.8S-25S rDNA genes
We have analyzed the phylogenetic and genomic relationships in the genus Setaria Beauv. including diploid and tetraploid species, by means of the molecular diversity of the 5S rDNA spacer and chromosomal organization of the 5S and 18S-5.8S-25S rDNA genes. PCR amplification of the 5S rDNA sequences gave specific patterns. All the species studied here share a common band of about 340 bp. An additional band of an approximately 300-bp repeat unit was found for Setaria verticillata and the Chinese accessions of Setaria italica and Setaria viridis. An additional band of 450 bp was found in the sole species Setaria faberii. Fluorescent in situ hybridization was used for physical mapping of the 5S …
Genome discrimination in progeny of interspecific hybrids between Brassica napus and Raphanus raphanistrum
International audience
Genomic in situ hybridization (GISH) discriminates between the A and the B genomes in diploid and tetraploid Setaria species
International audience
Southern and fluorescent in situ hybridization detect three RAPD-generated PCR products useful as introgression markers in Petunia
Fluorescent in situ hybridization (FISH) was used to reveal the intrachromosomal organization of 11 RAPD markers localized on the genetic map of Petunia hybrida. The cloned RAPD markers were analyzed by means of Southern hybridization to determine their level of sequence repetition and their specificity in different Petunia species with 2n=14 and 18 chromosomes. The same probes were then used in FISH experiments. Most of the RAPD clones studied showed high sequence repetition and no species specificity. Moreover, FISH analysis showed that these probes could belong to multilocus families as evidenced by the multiple FISH signals dispersed throughout the genome and present on every chromosome…