0000000000189343

AUTHOR

Manfred Nimtz

showing 3 related works from this author

7-O-acetyl-GD3 in human T-lymphocytes is detected by a specific T-cell-activating monoclonal antibody.

1995

The monoclonal antibody U5, which is a potent inducer of proliferation in human T-cells, was found to bind to an alkali-sensitive derivative of ganglioside GD3. Using immunochemical and spectroscopic methods, the structure of the U5 antigen was determined as 7-O-acetyl-GD3. The antibody U5 did not react with 9-O-acetyl-GD3 and bound severalfold more stronger to 7-O-acetyl-GD3 than to GD3. U5 is the first antibody known to detect preferentially 7-O-acetyl-GD3. Flow cytometric analysis showed that each major class of human leukocytes contained a significant fraction of cells binding the U5 antibody.

medicine.drug_classT cellT-LymphocytesImmunoblottingMolecular Sequence DataMonoclonal antibodyLymphocyte ActivationBiochemistryMass SpectrometryAntigenAntibody SpecificityGangliosidesBlocking antibodymedicineLeukocytesGanglioside GD3AnimalsHumansMolecular BiologyDirect fluorescent antibodybiologyChemistryAntibodies MonoclonalCell BiologyMolecular biology7-O-acetyl-GD3Kineticsmedicine.anatomical_structureMilkCarbohydrate Sequencebiology.proteinlipids (amino acids peptides and proteins)CattleFemaleAntibodyThe Journal of biological chemistry
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TCR signalling network organization at the immunological synapses of murine regulatory T cells.

2017

Regulatory T (Treg) cells require T-cell receptor (TCR) signalling to exert their immunosuppressive activity, but the precise organization of the TCR signalling network compared to conventional T (Tconv) cells remains elusive. By using accurate mass spectrometry and multi-epitope ligand cartography (MELC) we characterized TCR signalling and recruitment of TCR signalling components to the immunological synapse (IS) in Treg cells and Tconv cells. With the exception of Themis which we detected in lower amounts in Treg cells, other major TCR signalling components were found equally abundant, however, their phosphorylation-status notably discriminates Treg cells from Tconv cells. Overall, this s…

0301 basic medicineProteomicsImmunological SynapsesProteomeCD3ImmunologyReceptors Antigen T-Cellchemical and pharmacologic phenomenaBiologyT-Lymphocytes RegulatoryArticleImmunological synapse03 medical and health sciencesT-Lymphocyte SubsetsImmunology and AllergyAnimalsPhosphorylationReceptorCells CulturedCD86Mice Inbred BALB CZAP-70 Protein-Tyrosine KinaseZAP70T-cell receptorCD28hemic and immune systemsImmunological SynapsesCell biology030104 developmental biologyMicroscopy Fluorescencebiology.proteinFemaleSignal TransductionEuropean journal of immunology
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Synthesis and Magnetic Properties of Novel Azamacrocyclic LnIII, CuII, FeIII, and SrII Complexes and Conformational Analysis of the Ligands

2008

Hexaalkoxy-substituted azamacrocyclic metal complexes 1 · MXn [MXn = FeCl3, CuCl2, SrCl2, Ln(NO3)3; Ln = La, Pr, Eu, Ho, Er] with various chain lengths were prepared by a convergent approach using 1,2-bisalkoxy-4,5-diamines 3 and 4-alkoxy-pyridine-2,6-dicarbaldehydes 4 as key building blocks for template-assisted cyclocondensation. Metal complexes 1 · MXn were considered as potential metallomesogens. However, differential scanning calorimetry and optical polarizing microscopy of 1 · MXn did not reveal any mesomorphism. The magnetic susceptibility shows deviation from Curie-like behavior. Due to ligand field effects the effective magnetic moments are a function of the temperature. In order t…

Ligand field theoryMagnetic momentChemistryLigandCharge densityInfrared spectroscopyMagnetic susceptibilityInorganic ChemistryMetalCrystallographyComputational chemistryvisual_artvisual_art.visual_art_mediumDensity functional theoryZeitschrift für anorganische und allgemeine Chemie
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