Cultivable Fungal Endophytes in Roots, Rhizomes and Leaves of Posidonia oceanica (L.) Delile along the Coast of Sicily, Italy

The presence of endophytic fungi in the roots, rhizomes, and leaves of Posidonia oceanica was evaluated in different localities of the Sicilian coast. Samples of roots, rhizomes, and leaves were submitted to isolation techniques, and the obtained fungal colonies were identified by morphological and molecular (rRNA sequencing) analysis. Fungal endophytes occurred mainly in roots and occasionally in rhizomes and leaves. Lulwoana sp. was the most frequent of the isolated taxa, suggesting a strong interaction with the host. In addition, eight other fungal taxa were isolated. In particular, fungi of the genus Ochroconis and family Xylariaceae were identified as endophytes in healthy plants at al…

Sull’impiego di inoculi AM in ambito vivaistico

Kayaking performance is altered in mentally fatigued young elite athletes

Background The present study aimed to assess the impact of 60 min of a cognitive demanding task inducing mental fatigue (Stroop) on kayaking performance in young elite athletes. The second objective was to elucidate the effect of mental fatigue on performance in a population of young under-17 elite athletes of national. Methods Thirteen under-17 elite kayakers completed 60 min of an incongruent Stroop color-word test, or the equivalent time in a control condition in a cross-over study design. Afterwards, participants completed a 2000 m kayaking time trial in which power output, stroke rate and time at the end of 400, 800, 1200, 1600 and 2000 m were recorded. Physiological and perceptual mea…

Preliminary observations on fungal endophytic community and mycorrhizal status in symptomless Quercus ilex seedlings

Recently, a study on the Quercus ilex L. decay caused by biotic and abiotic agents has hypothesized alterations on endophytic and mycorrhizal fungal populations. Since holm oak is among the most representative Mediterranean species, that occurs widely in both urban and rural areas, in this work we preliminarly report these symbiotic fungal communities in asymptomatic seedlings, in order to compare these populations with those found in seedlings subjected to water and saline stress. Endophytic fungi were isolated from twigs and leaves and identified by morphological and molecular analysis. The ectomycorrhizal status was evaluated on root samples randomly selected to calculate the percentage …

Diversità della comunità ectomicorrizica presente in un impianto artificiale di conifere alloctone in Sardegna

Nel presente contributo sono riportati i risultati di uno studio sulla diversità della comunità fungina ectomicorrizica (ECM) presente in un impianto artificiale di conifere alloctone (Abies alba Mill., Pinus nigra Arnold e Cedrus atlantica (Endl.) Manetti ex Carrière), in cui è presente rinnovazione di A. alba e di Quercus ilex L., unica specie vegetale arborea autoctona presente nell’ambiente di studio. L’area d’indagine è localizzata a Tempio Pausania (Sardegna), sul monte Limbara in località Madonna della Neve (1262 s.l.m). Al suo interno sono stati individuati 5 plot omogenei, ed in ciascuno di essi sono stati prelevati frammenti radicali dalla base di esemplari adulti di A. alba, P. n…

Lulwoana sp., a dark septate endophyte (DSE) in roots of Posidonia oceanica (L.) Delile seagrass

Posidonia oceanica is the most common, widespread and important monocotyledon seagrass in the Mediterranean Basin, and hosts a large biodiversity of species, including microorganisms with key roles in the marine environment. In this study, we ascertain the presence of a fungal endophyte in the roots of P. oceanica growing on different substrata (rock, sand and matte) in two Sicilian marine meadows. Staining techniques on root fragments and sections, in combination with microscope observations, were used to visualise the fungal presence and determine the percentage of fungal colonisation (FC) in this tissue. In root fragments, statistical analysis of the FC showed a higher mean in roots anch…

Molecular characterization of citrus tristeza virus (CTV) isolates and detection of fungi associated with Citrange root-stock in Sicilian symptomless Citrus growes.

The first monitoring on the CTV occurrence in central-western Sicily carried out during spring 2011 in asymptomatic citrus groves, has allowed to report CTV- free citrus-growing areas (province of Caltanissetta and Trapani), the first focus in province of Agrigento and two new others in province of Palermo. Molecular analysis (RT-PCR of p18, p20 and p23 genes, single strand conformation polymorphism and partial sequencing) showed that the obtained CTV isolates had a high homology (99%) with mild CTV strains (T 30 and T 385). Moreover, survey on the genetic flow and analysis of synonymous/nonsynonymous, by MEGA 5.0 and DNAsp 5.0 programs, demonstrated the provenience of the new “tristeza” fo…

Descrizione delle micorrize del gruppo Thelephorales ed ecologia specifica delle specie più importanti trovate in un impianto di conifere alloctone in Sardegna

La famiglia delle Thelephoraceae include diverse specie di funghi basidiomiceti imenomiceti con corpi fruttiferi dalla forma assai variabile, coriacei o carnosi e a habitat tipicamente terricolo o lignicolo (saprotrofi, cariogeni e micorrizici, in prevalenza). A tale famiglia, la cui sistematica è ancora incerta, si attribuisce attualmente una decina di generi, fra i quali Thelephora, Tomentella, Tomentellopsis, Pseudotomentella e Lenzitopsis sono frequentemente riportati in letteratura come ectomicorrizici (ECM) in simbiosi con radici di piante forestali (aghi- e latifoglie), in vari ecosistemi. In particolare, studi dettagliati riguardano la descrizione morfologica delle ECM che essi inst…