0000000000190784

AUTHOR

Armando Gregorini

showing 5 related works from this author

Cytogenetics of the land snails Cantareus aspersus and C. mazzullii (Mollusca: Gastropoda: Pulmonata).

2004

A cytogenetic study was carried out on the chromosomes and nuclear DNA contents of the land snails Cantareus aspersus and C. mazzullii (Gastropoda: Pulmonata). Chromosomes were studied using Giemsa staining, banding methods and fluorescent in situ hybridization (FISH) with three repetitive DNA probes [18S rDNA, (GATA)n and (TTAGGG)n]. Results were very similar in the two species both showing (1) 54 bi-armed chromosomes [submetacentrics (SM) C metacentrics (M) C subtelocentrics (ST)]; (2) 10 terminal NORs after sequential application of rDNA FISH and silver staining; (3) uniform DNA fluorescence with CMA3 and DAPI staining and (4) genomic composition considerably enriched both in highly- and…

medicine.medical_specialtySnailsGeneral Physics and AstronomyPulmonataDNA RibosomalGiemsa stainSilver stainchemistry.chemical_compoundCytogeneticsSpecies SpecificityStructural BiologyGastropodamedicineAnimalsGeneral Materials ScienceIn Situ Hybridization FluorescenceRepetitive Sequences Nucleic AcidbiologyBase SequenceStaining and LabelingCytogeneticsCell BiologyDNATelomerebiology.organism_classificationMolecular biologyStainingNuclear DNAChromosome BandingchemistryKaryotypingDNAMicron (Oxford, England : 1993)
researchProduct

Molecular and biometric data on Carabus (Macrothorax) morbillosus Fabricius, 1792 (Coleoptera, Carabidae) from Mid Mediterranean areas

2022

The present study was carried out using molecular and biometric data of Carabus (Macrothorax) morbillosus from mid-Mediterranean areas to determine additional information on basal relationships among its representative subspecies. To this aim, two different kinds of approach were employed, including a morphometric analysis of four morphological parameters (i.e., elytra length, elytra width, pronotum length, pronotum width) of 128 specimens, and a Bayesian genetic analysis of 44 cytochrome oxidase subunit I (COI) partial sequences (i.e., 38 examined for the first time and six retrieved from GenBank database). Representative populations of C. (M.) morbillosus were sampled in four countries, n…

pronotumInsectaArthropodaSettore BIO/05 - ZoologiaCarabusBayesian analysisCOICarabinitaxonomyBayesian analysis biogeography carabids COI elytra morphometric analysis pronotum taxonomyCaraboideaAnimaliaCarabinaCarabus morbillosusEcology Evolution Behavior and Systematicsbiogeographyelytracarabidsmorphometric analysisCarabinaeBiotaColeopteraSettore AGR/11 - Entomologia Generale E ApplicataAnimal Science and ZoologyCarabidaeZooKeys
researchProduct

CD38 expression enhances sensitivity of lymphoma T and B cell lines to biochemical and receptor-mediated apoptosis

2006

CD38 has been widely characterised both as an ectoenzyme and as a receptor. In the present paper, we investigated the role of CD38 as possible modulator of apoptosis. CD38-positive (CD38(+)) and negative (CD38(-)) fractions, obtained by sorting CD38(+) cells from lymphoma T (Jurkat) and lymphoma B (Raji) and by transfecting lymphoma LG14 and myeloid leukemia K562 cell lines, were used. Cellular subpopulations were exposed to different triggers (H(2)O(2), UV-B, alpha-TOS and hrTRAIL) and the extent of apoptosis was determined by Annexin V-FITC/PI assay. Our data showed that, in lymphoma cells, propensity to apoptosis was significantly linked to CD38 expression and that, remarkably, such resp…

Lymphoma B-CellCD30Ultraviolet RaysTocopherolsApoptosisCD38BiologyLymphoma T-CellJurkat cellsTNF-Related Apoptosis-Inducing LigandJurkat Cellsimmune system diseasesAnnexinCell Line Tumorhemic and lymphatic diseasesmedicineHumansVitamin EAnnexin A5B cellhemic and immune systemsHydrogen PeroxideCell BiologyGeneral MedicineOligonucleotides AntisenseFlow CytometryADP-ribosyl Cyclase 1Antigens DifferentiationMolecular biologyBCL10medicine.anatomical_structureApoptosisAnnexin A5K562 CellsFluorescein-5-isothiocyanateCell Biology International
researchProduct

Karyotypes, Banding Patterns and Nuclear DNA Content inCrepidula unguiformisLamarck, 1822, andNaticarius stercusmuscarum(Gmelin, 1791) (Mollusca, Cae…

2009

ABSTRACT The chromosome complement and the nuclear DNA content in two caenogastropod species from the Mediterranean Sea, Crepidula unguiformis (Calyptraeidae) and Naticarius stercusmuscarum (Naticidae), were investigated by the application of both classical and molecular cytogenetic methods. Despite the constancy of haploid chromosome numbers (n = 17 in both species), C. unguiformis and N. stercusmuscarum show genome sizes amounting to 6.36 and 2.63 pg, respectively. Moreover, while N. stercusmuscarum resembles cytogenetically the other neotaenioglossan caenogastropods studied so far, C. unguiformis differs in: (i) number and location of rDNA clusters (ii), composition of telomeric repeats,…

GeneticsCaenogastropodaChromosomeNaticidaeKaryotypeBiologybiology.organism_classificationGenomeNuclear DNAEvolutionary biologyAnimal Science and ZoologyPloidyGenome sizeEcology Evolution Behavior and SystematicsMalacologia
researchProduct

Heterochromatin of the scarab beetle, Bubas bison (Coleoptera: Scarabaeidae) II. Evidence for AT-rich compartmentalization and a high amount of rDNA …

2005

An unexpected result arising from a previous characterization of the scarab beetle Bubas bison (Coleoptera: Scarabaeidae) heterochromatin was its unusual homogeneous reaction to different staining methods. In particular, silver stainability of heterochromatic ends of all chromosomes prevented identification of the number of rDNA transcriptionally active regions. Data formerly obtained using silver impregnation (Ag-NOR), C- G- and DAPI banding are here improved and completed by application of CMA(3) staining and rDNA FISH with the aim to investigate heterochromatin base composition and locate rDNA regions with respect to NOR-associated heterochromatin. Our results show that B. bison has a hi…

MaleHeterochromatinGeneral Physics and AstronomyDNA Ribosomalchemistry.chemical_compoundStructural BiologyHeterochromatinBotanyRNA Ribosomal 18SAnimalsGeneral Materials ScienceGeneIn Situ Hybridization FluorescenceScarabaeidaeStaining and LabelingbiologyRNAKaryotypeCell BiologyTelomereRibosomal RNAbiology.organism_classificationAT Rich SequenceChromosome BandingStainingColeopterachemistryEvolutionary biologyKaryotypingFemaleDNAMicron
researchProduct