Plant genotype: a lever in the interactions between plant and its associated rhizosphere microbiome

International audience; In the context of highly pressured agricultural production using low levels of inputs, cropping strategies could take advantage of existing plant-microbiome interactions that can improve both plant growth and health. We believe that plant genetics can provide some clues to better understand the mechanisms underlying microbiome recruitment by the plant.The aims of this work were (i) to assess plant genotype effect on the rhizosphere bacterial communities in relation to the plant nutritional strategies for a core collection of 155 genotypes of Medicago truncatula and (ii) to highlight the genetic determinisms potentially associated in these interactions. To achieve the…

Going back to roots: combining phenotyping, ecophysiology and molecular physiology

International audience

A new conceptual framework shows that plant genotype and soil nitrogen availability modify both plant nutritional strategies and diversity of their associated rhizosphere microbiome

In the context of sustainable agriculture, it is desirable to lower the input of mineral fertilizers. Therefore, we need to select ‘new’ genotypes that are both adapted to ‘low fertilizer inputs’ and more efficient in nutrient use. A better understanding of plant-microbe interactions under low input of fertilizers is now needed. Nevertheless, in microbial ecology, conceptual frameworks or models are used to analyse plant-microbe interactions but plant phenotype is currently viewed as a “black box”. We developed a new conceptual framework to study plant-microbe interactions using a multidisciplinary approach combining Microbial Ecology and Plant Ecophysiology.The links among plant genotype, …

Etude de l’interaction « Plante x Communauté microbienne du sol » chez l’espèce modèle Medicago truncatula

National audience

Crosstalks between soil microbial communities and a nitrogen fixing legume species (medicago truncatula) during its Vegetative Stage

International audience

Etude de l’interaction plante-communautés microbiennes de la rhizosphère chez l’espèce modèle Medicago truncatula par une approche multidisciplinaire : contribution à la réflexion sur le pilotage des interactions par la plante

The soil microbial communities can improve plant growth by increasing soil nutrient availability, thereby promoting their uptake by the plant. In an overall context of input reduction, the plant nutrition should be increasingly based on plant- rhizosphere microbial communities’ interactions. Yet, very few studies have examined the entire rhizosphere microbial communities in relationship with both plant genotype and phenotype. The aim of this thesis was to study the plant genotype effect on the rhizosphere microbial communities in relationship with the plant nutritional strategies. To do so, the plant-rhizosphere microbial communities’ interaction was assessed by a multidisciplinary approach…

Combining ecophysiological and microbial ecological approaches to assess interaction between genotypes of Medicago truncatula and the soil bacterial communities

International audience; Microbial communities can play a key role in sustainable agriculture by improving crop growth. Currently, a major stake is to study the effect of the plant genotype on the shaping of the soil microbial communities in relation to the plant nutrition. Methods: The links between the plant genotype and the rhizospheric bacterial communities were assessed at both structural and functional level using a multidisciplinary approach. On the one side, the genetic structure (Automated Ribosomial Intergenic Spacer Analysis (ARISA)) and diversity (454 pyrosequencing) of bacterial communities of the rhizosphere was first analysed. On the other side, using an ecophysiological model…

Combining molecular microbial ecology with ecophysiology and plant genetics for a better understanding of plant-microbial communities' interactions in the rhizosphere.

18 pages; International audience

Phenotyping, ecophysiology and molecular physiology to characterize plant root system architecture and plant-plant and plant-microorganisms interactions

International audience; Agriculture is facing the challenges to improve performance and crop adaptation to climate change towards reducing negative impacts of associated abiotic stresses on crop yield and contributing to its mitigation. Climate change comprises more frequent and ample abiotic stresses that plants have to cope with, such as drought, the most important limitation to yield stability and plant harvest product quality which often lead to hydromineral nutritional stress. The capacity of plants to extract nutrients and water from a given volume of soil depends on root system architecture and on intrinsic factors such as the production of root exudates and the efficiency for resour…

ILS3 highlighted nice results and challenging opportunities for innovative research on grain legume. Legume Perspective

From simple visualization to detailed understanding of plant and microbes interactions, using ‘pheno’ methods and models

International audience