0000000000194802
AUTHOR
H. Götz
Advanced Techniques of Micro-Analysis and Confocal Microscopy: Perspectives for Studying Chemical and Structural Changes at the Interface Between Restorative Materials and the Cavity Wall
Migration of trace amounts of elements and structural changes characterize the interface between immiscible substances. The contact zone among filling materials, saliva, and the cavity wall has the additional function of preventing the progress of leakage and subsequent caries. Difficulties in chemically and structurally analyzing the gradients of composition in an interface of microscopic dimensions characterize the experimental situation. The use of advanced techniques of instrumental micro-analysis and techniques of micro-visualization is our approach to the problem. With confocal laser scanning microscopy (CLSM), effects of the components of the filling material on the structure of the…
Fluoride-induced precipitates on enamel surface and subsurface areas visualised by electron microscopy and confocal laser scanning microscopy
The present study examined the enamel surface after in vitro topical treatments with a neutral 2% NaF solution. For minimising the risk of artefacts, samples were inspected without pre-treatment as fresh, naturally wet specimens by complementary techniques: variable pressure electron microscopy (VP-SEM) and confocal laser scanning microscopy (CLSM). VP-SEM provided information on the surface morphology, whereas CLSM allowed non-destructive visualisation of subsurface areas. Neutral NaF solutions induced globular precipitates on the enamel surfaces. If the globules formed may be described as "calcium fluoride like material", the additional information of this experiment is that, after intera…