Neuronal FGFR1 transactivation by inducing FGFR1/5-HT1A heteroreceptor complexes formation

There are no clear data on the molecular mechanism by which the hippocampal 5-HT transmission contributes to the neuroprotective and antidepressant effects of 5-HT uptake blockers. Previously, we revealed that a 5-HT1A agonist may induce phosphorylation of FGFR1 and ERK1/2 in rat hippocampus independent of FGF-2, suggesting transactivation of FGFR1 tyrosine kinase in the absence of neurotrophic factor binding. As extension of previous work, using BRET analysis and coimmunoprecipitation in cellular models, FGFR1-5-HT1A heteroreceptor complexes have been demonstrated and agonist modulation characterized. In vitro assays on ERK1/2 phosphorylation in HEK cells and primary hippocampal cultures h…

Serotonin receptor agonist treatment induces transactivation of fibroblast growth factor receptor-1 (FGFR1) tyrosine kinase in the rat hippocampal neurons

Over the past decade, many examples of activation of receptor tyrosine kinases in response to G-protein coupled receptor signaling have been reported, indicating that there are alternative modes of receptor tyrosine kinase activation (transactivation) in the absence of neurotrophic factor binding. In the present work, we aimed to examine if 5-HT receptor subtype activation may induce fibroblast growth factor receptor-1 (FGFR1) phosphorylation through transactivation of tyrosine kinase. The study has been performed in young adult rats treated with the selective 5-HT1A receptor agonist 8-OHDPAT at the dose of 0.4 mg/kg/i.p.. FGFR1 phosphorylation was evaluated by immunoprecipitation and weste…

Acute intermittent nicotine treatment counteracts the reduced proliferation of neuronal precursor cells of the subventricular zone (svz) in aged rat brain

Transmitter and ormone mediated neurotrophism in the brain : the role of nicotinic, gluco and mineralcorticoid receptors and their molecular mechanisms

Molecular mechanism of FGF-2 gene regulation by nicotinic receptors