Natural degassing activity of the geothermal system of Soussaki(Greece):Environmental impact and gas hazard issues.

Additive Manufacturing of Multi‐Scale Porous Soft Tissue Implants That Encourage Vascularization and Tissue Ingrowth

Medical devices, such as silicone-based prostheses designed for soft tissue implantation, often induce a suboptimal foreign-body response which results in a hardened avascular fibrotic capsule around the device, often leading to patient discomfort or implant failure. Here, it is proposed that additive manufacturing techniques can be used to deposit durable coatings with multiscale porosity on soft tissue implant surfaces to promote optimal tissue integration. Specifically, the “liquid rope coil effect”, is exploited via direct ink writing, to create a controlled macro open-pore architecture, including over highly curved surfaces, while adapting atomizing spray deposition of a silicone ink t…

Valutazione della produzione di sedimenti in un bacino forestato

Neural Crest-Derived Chondrocytes Isolation for Tissue Engineering in Regenerative Medicine

Chondrocyte transplantation has been successfully tested and proposed as a clinical procedure aiming to repair articular cartilage defects. However, the isolation of chondrocytes and the optimization of the enzymatic digestion process, as well as their successful in vitro expansion, remain the main challenges in cartilage tissue engineering. In order to address these issues, we investigated the performance of recombinant collagenases in tissue dissociation assays with the aim of isolating chondrocytes from bovine nasal cartilage in order to establish the optimal enzyme blend to ensure the best outcomes of the overall procedure. We show, for the first time, that collagenase H activity alone …

Indagine sperimentale di pieno campo sulla formazione dei rill

Monitoraggio dell'erosione rill a scala di parcella

Indagine di campo sul comportamento idraulico dei rill a scala di parcella

Field investigation on rilling in the experimental Sparacia area, South Italy

In this paper the results of a field investigation on rilling carried out in the experimental Sparacia area are reported. The measurements were made on a plot 6 m wide and 22 m long subjected to natural rainfalls. For ten rainfalls the total soil loss (interrill and rill erosion) was collected in a storage system consisting of two tanks arranged in series at the base of the plot. Rill morphology (rill length and cross-sections) was measured for five rainfall events, while the rill profile was surveyed for three events. First the contribution of each component (rill and interrill erosion) to total soil loss was established. Then the analysis allowed establishment of a power relationship betw…

Indagine regionale sul trasporto solido in sospensione dei corsi d'acqua siciliani

Isolation of Adult Rat Cardiomyocytes Using Recombinant Collagenases

Direct isolation of primary cells from tissues and organs allows for the maintenance of important cell characteristics and properties for in vitro studies and a plethora of biomedical applications. Dissociation of cells from the organ of interest is possible due to the enzymatic activity of collagenases. The choice and the dose of these enzymes is the critical step to obtain the maximum number of cells with intact structure and function. In this contest, Abiel collagenases class I (Col G) and class II (Col H) were synthesised using recombinant DNA technologies and their ability to degrade collagen in cell isolation from different tissues was tested. Examples of cells isolated with these enz…

Valutazione dell'indice mensile di aggressività delle piogge in Sicilia

3d collagen hydrogel promotes in vitro langerhans islets vascularization through ad-mvfs angiogenic activity

Adipose derived microvascular fragments (ad-MVFs) consist of effective vascularization units able to reassemble into efficient microvascular networks. Because of their content in stem cells and related angiogenic activity, ad-MVFs represent an interesting tool for applications in regenerative medicine. Here we show that gentle dissociation of rat adipose tissue provides a mixture of ad-MVFs with a length distribution ranging from 33–955 μm that are able to maintain their original morphology. The isolated units of ad-MVFs that resulted were able to activate transcriptional switching toward angiogenesis, forming tubes, branches, and entire capillary networks when cultured in 3D collagen type-…