0000000000205040

AUTHOR

Ranieri Domenici

showing 3 related works from this author

Forensic validation of Y-chromosome STR polymorphisms in Italy: the GE.F.I. collaborative database

2003

Abstract Haplotype data of 1176 Italian males from 10 regions were obtained as a part of a collaborative validation exercise. Individual data are available at http://www.gefi-forensicDNA.it .

GeneticsForensic scienceGeographyHaplotypeIndividual dataGeneral MedicineY chromosomeInternational Congress Series
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The molecular characterization of a depurinated trial DNA sample can be a model to understand the reliability of the results in forensic genetics

2014

The role of DNA damage in PCR processivity/fidelity is a relevant topic in molecular investigation of aged/forensic samples. In order to reproduce one of the most common lesions occurring in postmortem tissues, a new protocol based on aqueous hydrolysis of the DNA was developed in vitro. Twenty-five forensic laboratories were then provided with 3.0 μg of a trial sample (TS) exhibiting, in mean, the loss of 1 base of 20, and a molecular weight below 300 bp. Each participating laboratory could freely choose any combination of methods, leading to the quantification and to the definition of the STR profile of the TS, through the documentation of each step of the analytical approaches selected. …

DNA depurination; Forensic genetics; PCR fidelity; STR typing; Biochemistry; Clinical BiochemistryPCR fidelityGenotyping TechniquesDNA damageSample (material)Clinical BiochemistryDNA depurinationReproducibility of ResultForensic geneticsBiologyPolymerase Chain ReactionBiochemistryNOAnalytical Chemistrylaw.inventionDNA depurination; PCR fidelity; STR typing; forensic genetics.Settore MED/43 - Medicina LegalelawSettore BIO/13 - Biologia ApplicataGenotypeHumansSTR typingGenotyping TechniquesPolymerase chain reactionProtocol (science)GeneticsMedicine (all)Reproducibility of ResultsForensic geneticDNAAmpliconDNA FingerprintingDNA depurination; Forensic genetics; PCR fidelity; STR typingSettore BIO/18 - GeneticaDNA depurination Forensic genetics PCR fidelity STR typingDNA profilingSettore MED/03 - Genetica MedicaMicrosatellite RepeatGenotyping TechniqueDNA depurination; Forensic genetics; PCR fidelity; STR typing;Microsatellite RepeatsHuman
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Y-chromosome haplotypes in Italy: the GEFI collaborative database

2001

Abstract A sample of 1176 males from 10 Italian regions have been typed for DYS19, DYS389-I, DYS389-II, DYS390, DYS391, DYS392, DYS393, and DYS385. Individual haplotype data are available on line. A low degree of variation is present among regions. Use of this database is specifically recommended for forensic applications in Italy.

MalePopulation dataDatabases FactualDatabaseHaplotypePopulation geneticsSettore MED/43 - MEDICINA LEGALEcomputer.software_genreY chromosomePathology and Forensic MedicineGenetics PopulationGeographyHaplotypesItalyShort tandem repeatsY ChromosomePopulation dataHumansMicrosatelliteY haplotypeLawcomputerY-chromosome
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