0000000000207770
AUTHOR
Daniele Gallo
Correlation of Metabolic Syndrome with Redox Homeostasis Biomarkers: Evidence from High-Fat Diet Model in Wistar Rats
Metabolic Syndrome (MetS) is an extremely complex disease. A non-balanced diet such as high-fat diet (HFD) induces metabolic dysfunction that could modify redox homeostasis. We here aimed at exploring redox homeostasis in male Wistar rats, following 8 weeks of HFD, correlating the eventual modification of selected biomarkers that could be associated with the clinical manifestations of MetS. Therefore, we selected parameters relative to both the glucose tolerance and lipid altered metabolism, but also oxidative pattern. We assessed some biomarkers of oxidative stress i.e., thiols balance, lipid peroxidation and antioxidant barriers, via the use of specific biochemical assays, individuating e…
TRPV1 channels in nitric oxide-mediated signalling: insight on excitatory transmission in rat CA1 pyramidal neurons
Nitric oxide (NO) is a fascinating signalling molecule implicated in a plethora of biological functions, especially at the synaptic level. Exploring neurotransmission in the hippocampus could be instrumental in the individuation of putative targets for nitric-oxide mediated neuromodulation, especially in terms of the potential repercussions on fundamental processes i.e. synaptic plasticity and excitability-related phenomena. Among these targets, endovanilloid signalling constitutes an object of study since Transient Receptors Vanilloid type 1 (TRPV1) channels possess a NO-sensitive gate modulating its activation. Also, NO has been referred to as a mediator for numerous endocannabinoid effec…
TRPV1 channels as putative targets in the cannabinoid-mediated synaptic activity of hippocampal neurons
Endocannabinoids (eCBs) play a critical part in pathophysiological conditions rooted on neuronal excitability such as epilepsy. eCBs seem to be involved in neuroprotection, putatively acting on the cannabinoid receptor type 1 (CB1r), but also on Transient Receptor Potential Vanilloid type 1 channels (TRPV1). Indeed, CB1r and TRPV1 are involved in the transduction of stimuli at synaptic level, though exact molecular mechanisms are far from being unveiled. Thus, we aimed to investigate the role of CB1r/TRPV1 interplay in the rat hippocampal neurotransmission by whole-cell patch clamp technique to evaluate excitatory bioelectric activity in the CA1. We pharmacologically manipulated this pathwa…
Experimental evaluation of an hybrid communication system architecture for smart grid applications
In this paper a new communication system architecture is proposed for the development of a SCADA system which can remotely monitor and control intelligent electronic devices (IEDs) connected to the low voltage distribution networks, i.e low voltage smart meter (SM), interface protection devises (IPS). New distributed metering systems and interface devices, together with a proper communication infrastructure (HiperLAN and narrowband power line communication (NB-PLC) on low voltage network), have been developed to allow monitoring the whole power system and controlling the distributed generators of a Smart Grid also in the presence of harmonic distortions. In this way, the electrical energy d…
Compensation of Nonlinearity of Voltage and Current Instrument Transformers
This paper aims at characterizing and improving the metrological performances of current and voltage instrument transformers (CTs and VTs) in harmonic measurements in the power system. A theoretical analysis is carried out to demonstrate that, due to the iron core nonlinearity, CT and VT output signal is distorted even when the input signal is a pure sine wave. Starting from this analysis, a new method for CT and VT characterization and compensation is proposed. In a first step, they are characterized in sinusoidal conditions and the harmonic phasors of the distorted output are measured; in the second step, these phasors are used to compensate the harmonic phasors measured in normal operati…
Guanosine modulates K+ membrane currents in SH-SY5Y cells: involvement of adenosine receptors
AbstractGuanosine (GUO), widely considered a key signaling mediator, is implicated in the regulation of several cellular processes. While its interaction with neural membranes has been described, GUO still is an orphan neuromodulator. It has been postulated that GUO may eventually interact with potassium channels and adenosine (ADO) receptors (ARs), both particularly important for the control of cellular excitability. Accordingly, here, we investigated the effects of GUO on the bioelectric activity of human neuroblastoma SH-SY5Y cells by whole-cell patch-clamp recordings. We first explored the contribution of voltage-dependent K+ channels and, besides this, the role of ARs in the regulation…
Role of endocannabinoids and TRPV1 channels in the bioelectric activity of hippocampal neurons
It has been reported that endocannabinoid system is an important player in the regulation of neuronal bioelectrical activity, relying on receptor-mediated mechanisms. Amongst these, Cannabinoid receptor type 1 (CB1r) and Transient Receptor Potential Vanilloid type 1 (TRPV1) are both modulated by endocannabinoids, involved in the transduction of stimuli in the pre-synaptic neuron and prompt downstream pathways in the post-synaptic neuron. To investigate the role of CB1r/TRPV1 interplay, we applied whole-cell patch clamp technique to visualize the eventual variations in terms of membrane current and action potentials induced by pharmacological manipulation in rat hippocampal neurons. We modul…