0000000000207824

AUTHOR

Luis Serrano

showing 3 related works from this author

Bacterial antisense RNAs are mainly the product of transcriptional noise

2015

Most of the antisense transcripts in bacteria are the product of transcriptional noise derived from spurious promoters.

0301 basic medicineTranscription GeneticBacterial antisense RNAs030106 microbiologyinformation scienceBiologyGenomeTranscriptome03 medical and health sciencesSpecies SpecificityTranscription (biology)medicineLife Sciencenatural sciencesRNA AntisenseSystems and Synthetic BiologyResearch ArticlesGeneticsBiomoleculesMessenger RNASysteem en Synthetische BiologieMultidisciplinaryRNASciAdv r-articlesPromotersocial sciencesmedicine.diseaseequipment and supplieshealth care quality access and evaluationChloroplastRNA BacterialCardiovascular and Metabolic Diseasesbacterial antisense RNAsRNATranscriptomeTranscriptional noiseResearch ArticleScience Advances
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Combinatorial approaches: A new tool to search for highly structured β-hairpin peptides

2002

Here we present a combinatorial approach to evolve a stable β-hairpin fold in a linear peptide. Starting with a de novo -designed linear peptide that shows a β-hairpin structure population of around 30%, we selected four positions to build up a combinatorial library of 20 4 sequences. Deconvolution of the library using circular dichroism reduced such a sequence complexity to 36 defined sequences. Circular dichroism and NMR of these peptides resulted in the identification of two linear 14-aa-long peptides that in plain buffered solutions showed a percentage of β-hairpin structure higher than 70%. Our results show how combinatorial approaches can be used to obtain highly structured peptide s…

Models MolecularProtein FoldingCircular dichroismMagnetic Resonance SpectroscopyFold (higher-order function)Molecular Sequence DataPopulationPeptideComputational biologyBiologyProtein Structure SecondaryPeptide LibraryCombinatorial Chemistry TechniquesAmino Acid Sequenceeducationchemistry.chemical_classificationeducation.field_of_studyMultidisciplinaryCircular DichroismBiological SciencesCombinatorial chemistryTemplatechemistryDrug DesignDeconvolutionPeptidesProceedings of the National Academy of Sciences
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In vitro shoot organogenesis from excised immature cotyledons and microcuttings production in Stone Pine

1994

Adventitious buds were induced on isolated immature cotyledons of Pinus pinea L. in the presence of benzyladenine (BA). The response to different BA concentrations also depended upon the culture medium used (modified MS, SH and GD). A wide range of BA concentrations (5, 25 or 50 μM) can be applied to the GD and SH media, which are the media with the lower nitrogen content, without damaging effects. In the MS medium, which has the highest nitrogen concentration, the range of BA that can be applied was narrower and the highest BA concentration was lethal. The addition of indolebutyric acid (0.05, 0.25 or 0.5 μM) to the induction medium, decreased the response of cotyledons. The increase in th…

Sucrosefood.ingredientfood and beveragesHorticultureBiologychemistry.chemical_compoundTissue cultureMurashige and Skoog mediumfoodchemistryMicropropagationCytokininShootBotanyCotyledonExplant culturePlant Cell, Tissue and Organ Culture
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