0000000000208665
AUTHOR
F. Gary Toback
Direct nucleation of calcium oxalate dihydrate crystals onto the surface of living renal epithelial cells in culture
Direct nucleation of calcium oxalate dihydrate crystals onto the surface of living renal epithelial cells in culture. Background. The interaction of the most common crystal in human urine, calcium oxalate dihydrate (COD), with the surface of monkey renal epithelial cells (BSC-1 line) was studied to identify initiating events in kidney stone formation. Methods. To determine if COD crystals could nucleate directly onto the apical cell surface, a novel technique utilizing vapor diffusion of oxalic acid was employed. Cells were grown to confluence in the inner four wells of 24-well plates. At the start of each experiment, diethyloxalate in water was placed into eight adjacent wells, and the pla…
Sialic acid-containing glycoproteins on renal cells determine nucleation of calcium oxalate dihydrate crystals
Sialic acid-containing glycoproteins on renal cells determine nucleation of calcium oxalate dihydrate crystals. Background The interaction between the surfaces of renal epithelial cells and calcium oxalate dihydrate (COD), the most common crystal in human urine, was studied to identify critical determinants of kidney stone formation. Methods A novel technique utilizing vapor diffusion of oxalic acid was employed to nucleate COD crystals onto the apical surface of living cells. Confluent monolayers were grown in the inner 4 wells of 24-well culture plates. To identify cell surface molecules that regulate crystal nucleation, cells were pretreated with a protease (trypsin or proteinase K) to a…