0000000000212307
AUTHOR
Rosaria Tinnirello
Selezione di cloni più resistenti allo stress ossidativo dalla popolazione di cellule staminali mesoangioblasti
Autocrine role of extracellular Hsp70 in mesoangioblast migration capability
MMP2 synthesis in mouse mesoangioblast stem cells is highly regulated
Metalloproteasi nella biologia dei mesoangioblasti di topo
Cell clone selected from mab stem cell is more resistent to oxidative stress and retain some ability that could be useful for cell therapy.
Differentiation capability of clones of mouse mesoangioblast stem cells resistant to oxidative stress.
Hsp70 and NF-kB are involved in mouse mesoangioblast stem cells migration.
Hsp70 level regulates MMP2 expression in mesoangioblast stemj cells
Mesoangioblast stem cell population is non-omogeneous as revealed by transcriptome analysis after a severe oxidative stress.
Heat Shock Proteins in Multiple Sclerosis Pathogenesis: Friend or Foe?
Multiple Sclerosis is a complex chronic inflammatory, neurodegenerative disease conditioned by genetic, epigenetic and environmental factors. Main pathological features of MS include areas of focal demyelination of white matter characterized by gliosis, neuron and oligodendrocyte loss. Neurodegenerative as well as immune-mediated processes play a role in the pathogenesis of this disease. One of these immunogenic factors could be represented by the heat shock proteins. HSP exhibit cytoprotective and cytostimulatory effects due to their molecular chaperones role, in many brain model misfolding diseases such as Alzheimer’s and Parkinson’s diseases, whereas still no unambiguous results have bee…
La regolazione dell’espressione della MMP2 in mesoangioblasti è dipendente dai livelli della proteina Hsp70.
MMP2 expression in mouse mesoangioblast is dependent on Hsp70 level.
A sub-population of mesoangioblasts displays features of resistance and proliferation confirmed by transcriptome analysis.
The migration capability of mouse mesoangioblast stem cells depends on Hsp autocrine signalling.
Extracellular vesicles isolated by cerebrospinal fluid as biomarkers of inflammation in multiple sclerosis and inflammatory neurological diseases.
Meccanismo di rilascio di Hsp70 tramite vescicole di membrana e suo ruolo nella migrazione di cellule staminali di topo
Mouse mesoangioblast are vessel-derived stem cells which are able to differentiate in most mesodermal tissues, and have also the ability to migrate and cross the endothelial barrier. In our previous work we have demonstrated that these cells release in the extracellular medium membrane vesicles containing matrix metalloproteinases MMP-2 and MMP-9. We have also demonstrated that they express Hsp70 under basal growth condition. According to literature data, the aim of this study was to investigate the possible role of Hsp70 on MMPs regulation. To do this we compared mesoangioblasts A6 cell clone with two other cell clones: NM3, partially knockdown for Hsp70 expression and a cell clone overexp…
Mesoangioblast MMP2 regulation is dependent on Hsp70 level
Extracellular Hsp70 Enhances Mesoangioblast Migration via an Autocrine Signaling Pathway
Mouse mesoangioblasts are vessel-associated progenitor stem cells endowed with the ability of multipotent mesoderm differentiation. Therefore, they represent a promising tool in the regeneration of injured tissues. Several studies have demonstrated that homing of mesoangioblasts into blood and injured tissues are mainly controlled by cytokines/chemokines and other inflammatory factors. However, little is known about the molecular mechanisms regulating their ability to traverse the extracellular matrix (ECM). Here, we demonstrate that membrane vesicles released by mesoangioblasts contain Hsp70, and that the released Hsp70 is able to interact by an autocrine mechanism with Toll-like receptor …
Capacità di differenziamento di un clone cellulare da cellule staminali resistenti allo stress ossidativo.
Autophagy and apoptosis regolate survival of mesoangioblast stem cells subjected to oxidative stress
Extracellular membrane vesicles as a mechanism of cell-to-cell communication: advantages and disadvantages.
Microvesicles represent a newly identified mechanism of intercellular communication. Two different types of microvesicles have been identified: membrane-derived vesicles (EVs) and exosomes. EVs originate by direct budding from the plasma membrane, while exosomes arise from ectocytosis of multivesicular bodies. Recent attention has focused on the capacity of EVs to alter the phenotype of neighboring cells to make them resemble EV-producing cells. Stem cells are an abundant source of EVs, and the interaction between stem cells and the microenvironment (i.e., stem cell niche) plays a critical role in determining stem cell phenotype. The stem cell niche hypothesis predicts that stem cell number…