0000000000215209
AUTHOR
Miguel Hernández
miR‐200c and phospho‐AKT as prognostic factors and mediators of osteosarcoma progression and lung metastasis
Lung metastasis is the major cause of death in osteosarcoma patients. However, molecular mechanisms underlying this metastasis remain poorly understood. To identify key molecules related with pulmonary metastasis of pediatric osteosarcomas, we analyzed high-throughput miRNA expression in a cohort of 11 primary tumors and 15 lung metastases. Results were further validated with an independent cohort of 10 primary tumors and 6 metastases. In parallel, we performed immunohistochemical analysis of activated signaling pathways in 36 primary osteosarcomas. Only phospho-AKT associated with lower overall survival in primary tumors, supporting its role in osteosarcoma progression. CTNNB1 expression a…
Hypermethylation of apoptotic genes as independent prognostic factor in neuroblastoma disease
Neuroblastoma (NB) is an embryonal tumour of neuroectodermal cells, and its prognosis is based on patient age at diagnosis, tumour stage and MYCN amplification, but it can also be classified according to their degree of methylation. Considering that epigenetic aberrations could influence patient survival, we studied the methylation status of a series of 17 genes functionally involved in different cellular pathways in patients with NB and their impact on survival. We studied 82 primary NB tumours and we used methylation-specific-PCR to perform the epigenetic analysis. We evaluated the putative association among the evidence of hypermethylation with the most important NB prognostic factors, a…
Two independent epigenetic biomarkers predict survival in neuroblastoma.
Background Neuroblastoma (NB) is the most common extracranial pediatric solid tumor with a highly variable clinical course, ranging from spontaneous regression to life-threatening disease. Survival rates for high-risk NB patients remain disappointingly low despite multimodal treatment. Thus, there is an urgent clinical need for additional biomarkers to improve risk stratification, treatment management, and survival rates in children with aggressive NB. Results Using gene promoter methylation analysis in 48 neuroblastoma tumors with microarray technology, we found a strong association between survival and gene promoter hypermethylation (P = 0.036). Hypermethylation of 70 genes significantly …
Relation between adaptive learning actions and profiles of MOOCs users
The overcrowding and the heterogeneity of participants' profiles in a Massive Open Online Course (MOOC) are some of the main causes for a high dropout rate. International reports and research works points out the personalized learning as an important way to improve learning in any educational context. The information and communication technologies help to address adaptive technics in education through online courses. The specific characteristics of MOOCs point to the need to implement adaptive methodologies in MOOCs to increase the completion rates. This work presents a statistical analysis to find out in what aspects the condition of adaptivity, defined by the construct, is a preference of…
Intratumoral heterogeneity of DNA content in renal cell carcinoma and its prognostic significance
BACKGROUND A multiple sampling study was performed on 124 specimens of renal cell carcinomas to analyze the consistency and reliability of DNA measurements. The authors investigated intratumoral DNA heterogeneity and its role as a adverse prognostic factor for disease progression. METHODS DNA content was analyzed by flow cytometry on three different samples of the same tumor. The Cronbach α coefficient was used to assess the reliability and a Cox proportional hazards model was used to test the effect of DNA ploidy heterogeneity on time of disease progression. RESULTS The agreement among the DNA ploidy samples was high. The number of aneuploid findings increased significantly with the number…
Value of Deoxyribonucleic Acid Ploidy and Nuclear Morphometry for Prediction of Disease Progression in Renal Cell Carcinoma
AbstractPurpose: A retrospective study was performed on 108 patients with localized renal cell carcinoma (pT1 to 3a N0M0) to determine whether ploidy and nuclear morphometry are independent predictive factors in addition to stage and grade.Materials and Methods: Deoxyribonucleic acid (DNA) content was analyzed by flow cytometry and nuclear morphometry characterized by 5 nuclear descriptors. A Cox proportional hazards regression model was used to identify significant prognostic factors for disease progression.Results: A model combining tumor stage and grade, DNA ploidy and nuclear minor axis was chosen as optimal with risk of disease progression increased with increasing tumor stage and grad…
Miguel Hernández, Dirección General Servicios Penitenciarios de Cataluña
CriminologíaDuración: 29M
Salidas profesionales de la carrera de Criminología
CriminologíaDuración: 29M