0000000000218341

AUTHOR

Andreas Hombach

showing 2 related works from this author

Tuning tumor-specific T-cell activation: a matter of costimulation?

2002

Abstract The stimulation of a specific antitumor immune response, involving the recruitment of T cells and induction of T-cell effector functions, is an attractive possibility for cancer immunotherapy. In the past few years, advances in our understanding of the mechanisms of T-cell activation and costimulation have provided the basis for strategies to enhance antitumor immunity and break tolerance. These strategies include the equipment of tumor cells with costimulatory molecules such as B7, blockade of inhibitory signals on T cells (e.g. through cytotoxic T-lymphocyte antigen 4) and grafting of T cells with antigen-triggered, recombinant costimulatory receptors. Combining antigen-triggered…

ImmunoconjugatesT cellmedicine.medical_treatmentT-LymphocytesImmunologyBiologyLymphocyte ActivationImmunotherapy AdoptiveAbataceptCancer immunotherapyCD28 AntigensAntigens CDNeoplasmsmedicineImmunology and AllergyCytotoxic T cellHumansAntigens Tumor-Associated CarbohydrateCTLA-4 AntigenIL-2 receptorAntigen-presenting cellCD28ImmunotherapyAntigens Differentiationmedicine.anatomical_structureCTLA-4ImmunologyB7-1 AntigenTrends in immunology
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Tumor-specific T cell activation by recombinant immunoreceptors: CD3 zeta signaling and CD28 costimulation are simultaneously required for efficient …

2001

Abstract Recombinant immunoreceptors with specificity for the carcinoembryonic Ag (CEA) can redirect grafted T cells to a MHC/Ag-independent antitumor response. To analyze receptor-mediated cellular activation in the context of CD28 costimulation, we generated: 1) CEA+ colorectal tumor cells that express simultaneously B7-1 and B7-2, and 2) CEA-specific immunoreceptors that harbor intracellularly the signaling moities either of CD28 (BW431/26-scFv-Fc-CD28), CD3ζ (BW431/26-scFv-Fc-CD3ζ), or FcεRIγ (BW431/26-scFv-Fc-γ). By retroviral gene transfer, we grafted activated T cells from the peripheral blood with these immunoreceptors. T cells that express the FcεRIγ or CD3ζ signaling receptor lyse…

CD4-Positive T-LymphocytesCD3 ComplexT cellCD3T-LymphocytesImmunologyEpitopes T-Lymphocytechemical and pharmacologic phenomenaBiologyCD8-Positive T-LymphocytesMajor histocompatibility complexLymphocyte ActivationTransfectionEpitopeAntigenCD28 AntigensAntigens NeoplasmmedicineTumor Cells CulturedImmunology and AllergyHumansReceptors ImmunologicReceptorReceptors IgGCD28hemic and immune systemsMolecular biologyCoculture TechniquesRecombinant ProteinsCell biologyCarcinoembryonic Antigenmedicine.anatomical_structurebiology.proteinB7-1 AntigenInterleukin-2CD8Signal TransductionJournal of immunology (Baltimore, Md. : 1950)
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