0000000000218853

AUTHOR

Paola Ramoino

showing 2 related works from this author

Fast Inertia-Free Volumetric Light-Sheet Microscope

2017

Fast noninvasive three-dimensional (3D) imag-ing is crucial for quantitatively studying highly dynamic events ranging from flow cytometry to developmental biology. Light-sheet microscopy has emerged as the tool-of-choice for 3D characterization of rapidly evolving systems. However, to obtain a 3D image, either the sample or parts of the microscope are moved, limiting the acquisition speed. Here, we propose a novel inertia-free light-sheet-based scheme for volumetric imaging at high temporal resolution. Our approach comprises a novel combination of an acousto-optic scanner to produce tailored illumination and an acoustic-optofluidic lens, placed in the detection path to provide extended dept…

0301 basic medicineScanneracouto-optic devicesMaterials scienceMicroscopethree-dimensional microscopy01 natural sciencesAcouto-optic devices flow cytometry light-sheet microscopy three-dimensional microscopy Electronic Optical and Magnetic Materials Biotechnology Atomic and Molecular Physics and Optics Electrical and Electronic Engineeringlaw.invention010309 optics03 medical and health sciencesOpticslawAtomic and Molecular Physics0103 physical sciencesMicroscopyElectronicOptical and Magnetic MaterialsElectrical and Electronic Engineeringbusiness.industryflow cytometryRangingFrame rateAtomic and Molecular Physics and OpticsElectronic Optical and Magnetic MaterialsCharacterization (materials science)Lens (optics)acouto-optic devices; flow cytometry; light-sheet microscopy; three-dimensional microscopy; Electronic Optical and Magnetic Materials; Biotechnology; Atomic and Molecular Physics and Optics; Electrical and Electronic Engineering030104 developmental biologyLight sheet fluorescence microscopyand Opticsbusinesslight-sheet microscopyBiotechnologyACS Photonics
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A Novel Fast Volumetric Light Sheet Microscopy

2016

Fast noninvasive three-dimensional (3D) imaging is crucial for the quantitative understanding of highly dynamic biological processes. Over the last decades, several fluorescence microscopy techniques have been developed in order to provide a faster and deeper imaging of thick biological samples [1]. Within this framework, Light Sheet Fluorescence Microscopy (LSFM) has emerged as a powerful imaging tool for 3D imaging of thick samples ranging from single cells to entire animals [2,3].However, to obtain a 3D reconstruction either sample or microscope parts usually need to be moved limiting the acquisition speed and inducing possible interferences in volume recording. To solve this problem, he…

light sheet microscopyMicroscopeMaterials scienceImage qualitybusiness.industry3D reconstructionBiophysicsFrame ratelaw.inventionOpticslawLight sheet fluorescence microscopyTemporal resolutionMicroscopyDeconvolutionbusinessBiophysical Journal
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