6533b7d3fe1ef96bd1261611
RESEARCH PRODUCT
Fast Inertia-Free Volumetric Light-Sheet Microscope
Martí DuocastellaPeter SaggauAlberto DiasproAlberto DiasproPaolo BianchiniGiuseppe SancataldoGiuseppe SancataldoPaola Ramoinosubject
0301 basic medicineScanneracouto-optic devicesMaterials scienceMicroscopethree-dimensional microscopy01 natural sciencesAcouto-optic devices flow cytometry light-sheet microscopy three-dimensional microscopy Electronic Optical and Magnetic Materials Biotechnology Atomic and Molecular Physics and Optics Electrical and Electronic Engineeringlaw.invention010309 optics03 medical and health sciencesOpticslawAtomic and Molecular Physics0103 physical sciencesMicroscopyElectronicOptical and Magnetic MaterialsElectrical and Electronic Engineeringbusiness.industryflow cytometryRangingFrame rateAtomic and Molecular Physics and OpticsElectronic Optical and Magnetic MaterialsCharacterization (materials science)Lens (optics)acouto-optic devices; flow cytometry; light-sheet microscopy; three-dimensional microscopy; Electronic Optical and Magnetic Materials; Biotechnology; Atomic and Molecular Physics and Optics; Electrical and Electronic Engineering030104 developmental biologyLight sheet fluorescence microscopyand Opticsbusinesslight-sheet microscopyBiotechnologydescription
Fast noninvasive three-dimensional (3D) imag-ing is crucial for quantitatively studying highly dynamic events ranging from flow cytometry to developmental biology. Light-sheet microscopy has emerged as the tool-of-choice for 3D characterization of rapidly evolving systems. However, to obtain a 3D image, either the sample or parts of the microscope are moved, limiting the acquisition speed. Here, we propose a novel inertia-free light-sheet-based scheme for volumetric imaging at high temporal resolution. Our approach comprises a novel combination of an acousto-optic scanner to produce tailored illumination and an acoustic-optofluidic lens, placed in the detection path to provide extended depth-of-field. Such a combination enables unsurpassed 3D imaging speeds up to 200 volumes per second. The volumetric imaging speed is limited only by the acquisition frame rate of the camera and the desired signal-to- noise ratio. Our volumetric microscope allows for invariant acquisition along the detection axis, avoiding extensive processing or complex deconvolution methods to restore image quality.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2017-06-22 | ACS Photonics |