0000000000219274

AUTHOR

Robin Leake

showing 4 related works from this author

Do multiple oestrogen receptor assays give significant additional information for the management of breast cancer?

1989

In 101 breast cancer patients, measurement of oestrogen receptor status in multiple biopsies across a tumour reveals a highly significant difference in the proportion of patients remaining either disease-free (P less than 0.04) or alive (P less than 0.005), when those with uniformly receptor positive (++) primary tumours are matched with clinically comparable patients whose tumours were homogeneously receptor negative (--). Mean follow-up time was 85 months. The prognostic value of this discriminant is particularly striking in the 53 patients with involved nodes at presentation. Of these, 13 were (++) and seven remain alive of whom six are disease-free, whereas 24 of the 29 (--) patients ar…

OncologyCancer Researchmedicine.medical_specialtymedicine.drug_classMammary glandBreast NeoplasmsBiologyBreast cancerInternal medicineClinical informationmedicineHumansOestrogen receptorReceptorSignificant differencemedicine.diseasePrognosisMenopauseEndocrinologymedicine.anatomical_structureOncologyReceptors EstrogenEstrogenFemaleMenopauseResearch ArticleBritish journal of cancer
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Steroid-growth factor interaction in human prostate cancer. 2. Effects of transforming growth factors on androgen metabolism of prostate cancer cells

1996

The ability of human prostate cancer cells to metabolize androgens was assessed through administration of physiological concentration (0.5-10 nM) of tritiated testosterone (T) as precursor and one-step analysis of both T degradation and products' formation by reverse-phase HPLC and on-line radioactive detection after either 24 h or 72 h incubation. Overall, different prostate cancer cells degraded T quite differently, favoring alternatively reductive or oxidative metabolic pathways. In particular, both LNCaP and DU145 cells retained high levels of unconverted T, with a limited production of androstenedione and its 17-keto derivatives and relatively high amounts of dihydrotestosterone (DHT) …

Malemedicine.medical_specialtymedicine.drug_classClinical BiochemistryBiologyurologic and male genital diseasesBiochemistrychemistry.chemical_compoundEndocrinologyDU145Transforming Growth Factor betaInternal medicineLNCaPTumor Cells CulturedmedicineHumansMolecular BiologyTestosteronePharmacologyAndrosteroneOrganic ChemistryProstatic NeoplasmsTransforming Growth Factor alphaAndrogenEndocrinologychemistryDihydrotestosteroneCancer cellAndrogensmedicine.drugTransforming growth factorSteroids
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Steroid-growth factor interaction in human prostate cancer. 1. Short-term effects of transforming growth factors on growth of human prostate cancer c…

1994

In order to better define potential mechanisms of growth regulation in human prostate cancer cells, we have compared biological responses (such as short-term response to both transforming growth factor alpha and beta; TFG alpha and TFG beta) in relation to hormone sensitivity of LNCaP, DU145, and PC3 cells. Androgen receptor (AR) and epidermal growth factor receptor (EGF-R) content of each cell line was also investigated. In addition, expression of EGF, TGF alpha, and TGF beta was evaluated through immunofluorescent staining. Growth of androgen non-responsive PC3 cells was stimulated by TGF alpha (about 35%) and inhibited by TGF beta (more than 50%), with respect to controls, after 48 h exp…

Malemedicine.medical_specialtyTime Factorsmedicine.medical_treatmentClinical BiochemistryFluorescent Antibody Techniqueurologic and male genital diseasesBiochemistryProstate cancerEndocrinologyDU145Transforming Growth Factor betaInternal medicineLNCaPTumor Cells CulturedmedicineHumansReceptors Growth FactorEpidermal growth factor receptorMolecular BiologyPharmacologybiologyGrowth factorOrganic ChemistryProstatic NeoplasmsTransforming Growth Factor alphamedicine.diseaseAndrogen receptorEndocrinologyReceptors AndrogenCancer cellAndrogensbiology.proteinCell DivisionTransforming growth factorSteroids
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Inhibition of intrinsic protein tyrosine kinase activity of EGF-receptor kinase complex from human breast cancer cells by the marine sponge metabolit…

1990

1. (+)-Aeroplysinin-1, a naturally occurring tyrosine metabolite from the marine sponge Verongia aerophoba, was found to inhibit the phosphorylation of lipocortin-like proteins by a highly purified preparation of the epidermal growth factor (EGF) receptor-tyrosine protein kinase complex from MCF-7 breast carcinoma cells. 2. (+)-Aeroplysinin-1 blocked the EGF-dependent proliferation of both MCF-7 and ZR-75-1 human breast cancer cells and inhibited the ligand-induced endocytosis of the EGF receptor in vitro. 3. Treatment with aeroplysinin-1 in the concentration range at 0.25-0.5 microM resulted in a time- and dose-dependent total tumor cell death in vitro. 4. At a 10-fold higher concentration…

Protein kinase complexAcetonitrilesTime FactorsPhysiologyBlotting WesternBreast NeoplasmsBiologyBiochemistrySubstrate SpecificityMiceEpidermal growth factorCyclohexenesTumor Cells CulturedAnimalsHumansPhosphorylationTyrosineMolecular BiologyDose-Response Relationship DrugKinaseGeneral MedicineProtein-Tyrosine KinasesMolecular biologyPoriferaErbB ReceptorsBiochemistryCell cultureCancer cellPhosphorylationCalciumTyrosine kinaseCell DivisionComparative Biochemistry and Physiology Part B: Comparative Biochemistry
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