Preliminary Studies for the Preparation of Casein-loaded Liposomes to Inhibit Aβ1-40 Fibrillogenesis

αs1-Casein is a natural protein which constitutes the most prevalent form of casein in bovine milk. αs1-Casein is amphiphilic, almost unfolded, and consist of two highly hydrophobic zones separated by an hydrophilic region. Previous studies have demonstrated the ability of αs1-Casein to inhibit in vitro the nucleation phase of amyloid β-peptide (Aβ) fibrillogenesis by sequestering Aβ species on its surface. One of the main hallmark for Alzheimer Disease (AD) is the extracellular deposition in brain tissues of proteinaceous plaques, rich of well-ordered Aβ peptide amyloid aggregates. Although such an evidence, oligomeric intermediates in the fibrillogenesis have been discovered to be the mos…