0000000000223404

AUTHOR

Belén López-garcía

showing 3 related works from this author

Stabilisation of mixed peptide/lipid complexes in selective antifungal hexapeptides

2004

AbstractThe design of antimicrobial peptides could have benefited from structural studies of known peptides having specific activity against targetmicrobes, but not toward other microorganisms. We have previously reported the identification of a series of peptides (PAF-series) activeagainst certain postharvest fungal phytopathogens, and devoid of toxicity towards E. coli and S. cerevisiae [Lo´pez-Garci´a et al. Appl.Environ. Microbiol. 68 (2002) 2453]. The peptides inhibited the conidia germination and hyphal growth. Here, we present a comparativestructural characterisation of selected PAF peptides obtained by single-amino-acid replacement, which differ in biological activity. Thepeptides w…

Hyphal growthCircular dichroismAntifungal AgentsProtein ConformationStereochemistryFungicideAntimicrobial peptidesBiophysicsPeptideMicrobial Sensitivity TestsBiochemistryMembrane LipidsmedicinePostharvestMicelleschemistry.chemical_classificationMembranesCircular DichroismBiological activityCell BiologyPlantsSpectrometry FluorescenceConformational analysisMembraneEnergy TransferMechanism of actionBiochemistrychemistryDrug DesignSpecific activitymedicine.symptomAntimicrobial peptidePeptide–lipid interactionOligopeptidesBiochimica et Biophysica Acta (BBA) - Biomembranes
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Comparison of the activity of antifungal hexapeptides and the fungicides thiabendazole and imazalil against postharvest fungal pathogens

2003

8 pages, 2 figures, 3 tables.-- PMID: 14623382 [PubMed].-- Printed version published Dec 31, 2003.

CitrusAntimicrobial peptidesCitrus fruitsMicrobial Sensitivity TestsMicrobiologyMicrobiologyMinimum inhibitory concentrationRhizopusFood PreservationThiabendazolePostharvestFungicidesPenicillium digitatumDose-Response Relationship DrugbiologyImidazolesPenicilliumfood and beveragesGeneral MedicineAlternariabiology.organism_classificationAntimicrobialFungicides IndustrialFungicideFruitPenicilliumAntimicrobial peptidesOligopeptidesFood ScienceInternational Journal of Food Microbiology
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Identification of Novel Hexapeptides Bioactive against Phytopathogenic Fungi through Screening of a Synthetic Peptide Combinatorial Library

2002

The purpose of the present study was to improve the antifungal activity against selected phytopathogenic fungi of the previously identified hexapeptide PAF19. We describe some properties of a set of novel synthetic hexapeptides whose D-amino acid sequences were obtained through screening of a synthetic peptide combinatorial library in a positional scanning format. As a result of the screening, 12 putative bioactive peptides were identified, synthesized, and assayed. The peptides PAF26 (Ac-rkkwfw-NH(2)), PAF32 (Ac-rkwhfw-NH(2)), and PAF34 (Ac-rkwlfw-NH(2)) showed stronger activity than PAF19 against isolates of Penicillium digitatum, Penicillium italicum, and Botrytis cinerea. PAF26 and PAF3…

Antifungal AgentsMicrobial Sensitivity TestsApplied Microbiology and BiotechnologyMelittinPenicillium italicumMicrobiologyAntifungal peptidechemistry.chemical_compoundFusariumPeptide LibraryFusarium oxysporummedicineEnzymology and Protein EngineeringPeptide libraryPhytopathogenic fungiPlant DiseasesBotrytis cinereaPenicillium digitatumEcologybiologyPenicilliumfood and beveragesBiological activitybiology.organism_classificationmedicine.drug_formulation_ingredientBiochemistrychemistryPenicillium expansumPeptidesOligopeptidesHexapeptide PAF19Food ScienceBiotechnology
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