0000000000223521
AUTHOR
Haitham Al-haj
Low level 809-nm diode laser-induced in vitro stimulation of the proliferation of human gingival fibroblasts
Background and Objective The authors investigated the effects of low level laser irradiation on the proliferation rate of human gingival fibroblasts (HGF) in vitro. Study Design/Materials and Methods HGF were obtained from gingival connective tissue explants and cultured under standard conditions. 110 cell cultures in their logarithmic growth phase were spread on 96-well tissue culture plates and were irradiated at energy fluences of 1.96–7.84 J/cm2. Another 110 cultures served as control. An 809-nm semiconductor laser operated at a power output of 10 mW in the cw-mode was used. The time of exposure varied between 75 and 300 seconds. Laser treatment was performed alternatively once, twice, …
Intrapulpal temperature changes during root surface irradiation with an 809-nm GaAlAs laser
The aim of this study was to explore, in vitro, whether the irradiation of human root surfaces with a diode laser might induce nonphysiologic intrapulpal temperature elevations and, therefore, jeopardize pulp vitality.The pulps were removed from human maxillary and mandibular incisors extracted for periodontal reasons. The root canals were enlarged to an apical size #60 file. The teeth were radiographed with standard dental films and a millimeter grid to determine root thickness. The thickness of dentin between the root surface and the pulp in the irradiation areas was 1, 2, and 3 mm. To determine intrapulpal temperature changes during laser irradiation, 0.5-mm K-type thermocouples were ins…