0000000000223809
AUTHOR
P. Sáenz-gonzález
On-line in-tube solid phase microextraction coupled to capillary liquid chromatography-diode array detection for the analysis of caffeine and its metabolites in small amounts of biological samples.
Abstract In-tube solid phase microextraction (IT-SPME) coupled on-line to capillary liquid chromatography with diode array detection provides a simple and fast analytical methodology for the simultaneous quantitation of caffeine and its three primary metabolites (theobromine, paraxanthine and theophylline) in micro samples of serum, saliva and urine matrices. The sample amount required for one analysis was only 2.5 μL of saliva, 6.25 μL of serum or 40 μL of urine, a requirement for its implementation in a hospital laboratory for preterm newborns, where sample availability is a major problem. In standard conditions, 25 μL of diluted saliva or serum (or 100 μL of urine) were processed by IT-S…
Nursing care in therapeutic hypothermia in neonates with hypoxic-ischaemic encephalopathy. Review of the literature
Abstract Introduction Hypoxic-ischaemic encephalopathy is one of the main causes of neurological damage in the new-born. Therapeutic hypothermia is the current treatment to reduce mortality and disability in new-borns with this condition. Objective To identify nursing care in new-borns with severe to moderate EHI, treated with active therapeutic hypothermia. Materials and methods A review of the scientific literature was carried out in different databases (PubMed, Lilacs, IBECS, Cinhal, OvidSP, Cuiden, Embase and Cochrane Plus) over the last five years. The documentary assessment was carried out by peers and the quality was evaluated using the CEBM and GRADE scales. Results Of the 22 articl…
Cuidados enfermeros a neonatos con encefalopatía hipóxico-isquémica en hipotermia terapéutica. Revisión bibliográfica
Resumen Introduccion La encefalopatia hipoxico-isquemica es una de las principales causas de dano neurologico en el neonato. Actualmente, la hipotermia terapeutica es el tratamiento de eleccion para reducir la mortalidad y la discapacidad en los ninos que presentan esta patologia. Objetivo Identificar los cuidados enfermeros en recien nacidos con encefalopatia hipoxico-isquemica moderada a severa tratados con hipotermia terapeutica activa. Materiales y metodos Se realizo una revision de la literatura cientifica en diferentes bases de datos (PubMed, Lilacs, IBECS, Cinhal, OvidSP, Cuiden, Embase y Cochrane Plus) durante los ultimos 5 anos. La valoracion documental se realizo por pares y la ca…