0000000000223931

AUTHOR

Anke Busch

showing 3 related works from this author

Checkpoint adaptation in recombination-deficient cells drives aneuploidy and resistance to genotoxic agents.

2020

Abstract Human cancers frequently harbour mutations in DNA repair genes, rendering the use of DNA damaging agents as an effective therapeutic intervention. As therapy-resistant cells often arise, it is important to better understand the molecular pathways that drive resistance in order to facilitate the eventual targeting of such processes. We employ recombination-defective diploid yeast as a model to demonstrate that, in response to genotoxic challenges, nearly all cells eventually undergo checkpoint adaptation, resulting in the generation of aneuploid cells with whole chromosome losses that have acquired resistance to the initial genotoxic challenge. We demonstrate that adaptation inhibit…

Genome instabilitySaccharomyces cerevisiae ProteinsDNA RepairDNA repairAneuploidySaccharomyces cerevisiaeBiologyBiochemistryGenomic Instabilitychemistry.chemical_compoundGene Knockout TechniquesDrug Resistance FungalmedicineCytotoxicityMolecular BiologyRecombination GeneticSirolimusCell BiologyCell Cycle Checkpointsmedicine.diseaseAneuploidyPhenotypeDiploidyCell biologyRad52 DNA Repair and Recombination ProteinchemistryAdaptationPloidyDNADNA repair
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Ythdf is a N6‐methyladenosine reader that modulates Fmr1 target mRNA selection and restricts axonal growth in Drosophila

2021

Abstract N6‐methyladenosine (m6A) regulates a variety of physiological processes through modulation of RNA metabolism. This modification is particularly enriched in the nervous system of several species, and its dysregulation has been associated with neurodevelopmental defects and neural dysfunctions. In Drosophila, loss of m6A alters fly behavior, albeit the underlying molecular mechanism and the role of m6A during nervous system development have remained elusive. Here we find that impairment of the m6A pathway leads to axonal overgrowth and misguidance at larval neuromuscular junctions as well as in the adult mushroom bodies. We identify Ythdf as the main m6A reader in the nervous system,…

Nervous systemCancer ResearchAdenosineMessengerRNA-binding proteinBiologyArticleGeneral Biochemistry Genetics and Molecular BiologyFragile X Mental Retardation Protein03 medical and health scienceschemistry.chemical_compound0302 clinical medicinemedicineAnimalsDrosophila ProteinsFmr1; RNA modification; Ythdf; m6A; nervous systemRNA MessengerFmr1Molecular BiologyDrosophila030304 developmental biologyNeurons0303 health sciencesGeneral Immunology and MicrobiologyProteomics and Chromatin BiologyGeneral Neurosciencenervous systemRNA-Binding ProteinsTranslation (biology)Articlesm6AProtein Biosynthesis & Quality ControlRNA modificationYthdfbiology.organism_classificationRNA BiologyFMR1Fmr1; RNA modification; Ythdf; m6A; nervous system; Adenosine; Animals; Axons; Drosophila Proteins; Drosophila melanogaster; Fragile X Mental Retardation Protein; Neurons; RNA Messenger; RNA-Binding ProteinsAxonsCell biologyDrosophila melanogastermedicine.anatomical_structurechemistryMushroom bodiesRNATarget mrnaN6-Methyladenosine030217 neurology & neurosurgeryNeuroscienceThe EMBO Journal
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Checkpoint adaptation in repair-deficient cells drives aneuploidy and resistance to genotoxic agents

2018

AbstractHuman cancers frequently harbour mutations in DNA repair genes, rendering the use of DNA damaging agents as an effective therapeutic intervention. As therapy-resistant cells often arise, it is important to better understand the molecular pathways that drive resistance in order to facilitate the eventual targeting of such processes. We employ repair-defective diploid yeast as a model to demonstrate that, in response to genotoxic challenges, nearly all cells eventually undergo checkpoint adaptation, resulting in the generation of aneuploid cells with whole chromosome losses that have acquired resistance to the initial genotoxic challenge. We demonstrate that adaptation inhibition, eit…

DNA repairAneuploidyBiologymedicine.diseasePhenotypeYeastCell biologychemistry.chemical_compoundchemistrymedicinePloidyAdaptationCytotoxicityDNA
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