Sedimentary Ancient DNA (sedaDNA) Reveals Fungal Diversity and Environmental Drivers of Community Changes throughout the Holocene in the Present Boreal Lake Lielais Svētiņu (Eastern Latvia)
Fungi are ecologically important in several ecosystem processes, yet their community composition, ecophysiological roles, and responses to changing environmental factors in historical sediments are rarely studied. Here we explored ancient fungal DNA from lake Lielais Svētiņu sediment throughout the Holocene (10.5 kyr) using the ITS metabarcoding approach. Our data revealed diverse fungal taxa and smooth community changes during most of the Holocene with rapid changes occurring in the last few millennia. More precisely, plankton parasitic fungi became more diverse from the Late Holocene (2–4 kyr) which could be related to a shift towards a cooler climate. The Latest Holocene (~2 kyr) showed …
Holocene fires in the central European lowlands and the role of humans
International audience; A major debate concerns the questions of when and to what extent humans affected regional landscapes, especially land cover and associated geomorphological dynamics, significantly beyond natural variability. Fire is both, a natural component of many climate zones and ecosystems around the globe and also closely related to human land cover change. Humans clearly affected natural fire regimes and landscapes in the most recent centuries, acting as prime ignition triggers and later fire suppressors, while Holocene trends in sedimentary charcoal have been mainly associated with climatic factors and partly with Neolithic land cover change. However, little is known since wh…
Palaeoenvironmental evidence for the impact of the crusades on the local and regional environment of medieval (13th–16th century) northern Latvia, eastern Baltic
This paper evaluates the impact of the crusades on the landscape and environment of northern Latvia between the 13th–16th centuries (medieval Livonia). The crusades replaced tribal societies in the eastern Baltic with a religious state (Ordenstaat) run by the military orders and their allies, accompanied by significant social, cultural and economic developments. These changes have previously received little consideration in palaeoenvironmental studies of past land use in the eastern Baltic region, but are fundamental to understanding the development and expansion of a European Christian identity. Sediment cores from Lake Trikāta, located adjacent to a medieval castle and settlement, were s…
Holocene fire activity during low-natural flammability periods reveals scale-dependent cultural human-fire relationships in Europe
Abstract Fire is a natural component of global biogeochemical cycles and closely related to changes in human land use. Whereas climate-fuel relationships seem to drive both global and subcontinental fire regimes, human-induced fires are prominent mainly on a local scale. Furthermore, the basic assumption that relates humans and fire regimes in terms of population densities, suggesting that few human-induced fires should occur in periods and areas of low population density, is currently debated. Here, we analyze human-fire relationships throughout the Holocene and discuss how and to what extent human-driven fires affected the landscape transformation in the Central European Lowlands (CEL). W…
Investigating the impact of anthropogenic land use on a hemiboreal lake ecosystem using carbon/nitrogen ratios and coupled-optical emission spectroscopy
Anthropogenic impacts on lake ecosystems have increased substantially towards the present. However, the strength and timing in most cases are not evaluated in detail, missing valuable information on the response and recovery of an aquatic system. In this study, we use the sediment total organic carbon/total nitrogen ratio (C/N) and inductively coupled-optical emission spectroscopy (ICP-OES) elements and the available information about the biological processes to explore anthropogenic land use impact on the lake ecosystem. As a case study we selected a hemiboreal lake Trikātas (Latvia, NE Europe). The Pearson correlation was used to statistically test the correlations of all variables. Our r…
Broadleaf deciduous forest counterbalanced the direct effect of climate on Holocene fire regime in hemiboreal/boreal region (NE Europe)
Abstract Disturbances by fire are essential for the functioning of boreal/hemiboreal forests, but knowledge of long-term fire regime dynamics is limited. We analysed macrocharcoal morphologies and pollen of a sediment record from Lake Lielais Svētiņu (eastern Latvia), and in conjunction with fire traits analysis present the first record of Holocene variability in fire regime, fuel sources and fire types in boreal forests of the Baltic region. We found a phase of moderate to high fire activity during the cool and moist early (mean fire return interval; mFRI of ∼280 years; 11,700–7500 cal yr BP) and the late (mFRI of ∼190 years; 4500–0 cal yr BP) Holocene and low fire activity (mFRI of ∼630 y…
Towards understanding the abundance of non-pollen palynomorphs : A comparison of fossil algae, algal pigments and sedaDNA from temperate lake sediments
Given the increased interest in non-pollen palynomorphs (microscopic objects other than pollen identified from pollen slides) in palaeoecological studies, it is necessary to seek a deeper understanding of the reliability of these results. We combined quantitative information of algal pigments and sedimentary ancient DNA (sedaDNA) of phylotaxonomical resolution to validate the richness and abundance of fossil algae in the sediment of a small temperate lake. For the first time, fossil and sedaDNA algae data were combined in a composite data-set and used to reconstruct algae turnover rates over the last 14,500 years. This comparison serves as both an example of howfossil algae can be used to a…
From microbial eukaryotes to metazoan vertebrates: Wide spectrum paleo-diversity in sedimentary ancient DNA over the last ~14,500 years
Most studies that utilize ancient DNA have focused on specific groups of organisms or even single species. Instead, the whole biodiversity of eukaryotes can be described using universal phylogenetic marker genes found within well-preserved sediment cores that cover the post-glacial period. Sedimentary ancient DNA samples from Lake Lielais Svētiņu, eastern Latvia, at a core depth of 1,050 cm in ~150 year intervals were used to determine phylotaxonomy in domain Eukaryota. Phylotaxonomic affiliation of >1,200 eukaryotic phylotypes revealed high richness in all major eukaryotic groups-Alveolata, Stramenopiles, Cercozoa, Chlorophyta, Charophyta, Nucletmycea, and Holozoa. The share of organisms t…
Environmental drivers and abrupt changes of phytoplankton community in temperate lake Lielais Svētiņu, Eastern Latvia, over the last Post-Glacial period from 14.5 kyr
Understanding the long-term dynamics of ecological communities on the centuries-to-millennia scale is important for explaining the emergence of present-day biodiversity patterns and for predicting possible future scenarios. Fossil pigments and ancient DNA present in various sedimentary deposits can be analysed to study long-term changes in ecological communities. We analysed recent compilations of data, including fossil pigments, microfossils, and molecular inventories from the sedimentary archives, to understand the impact of gradual versus abrupt climate changes on the ecosystem status of a regional model lake over the last ~14.5 kyr. Such long and complete paleo-archives are scarce in No…
Drivers of change and ecosystem status in a temperate lake over the last Post-Glacial period from 14.5 kyr
AbstractUnderstanding the long-term dynamics of ecological communities on the centuries-to-millennia scale is important for explaining present-day biodiversity patterns. Placing these patterns in a historical context could yield reliable tools for predicting possible future scenarios. Paleoarchives of macro+ and micro-fossil remains, and most importantly biomarkers such as fossil pigments and ancient DNA present in various sedimentary deposits, allow long term changes in ecological communities to be analysed. We use recent compilations of data including fossil pigments, metabarcoding of sedimentary ancient DNA and microfossils together with data analysis to understand the impact of gradual …
Large herbivore population and vegetation dynamics 14,600–8300 years ago in central Latvia, northeastern Europe
Abstract This study seeks to explain how the large herbivore (large vertebrate, megafauna – terrestrial taxa with adults > 45 kg) population density changed during abrupt postglacial climate and environmental change. The Lateglacial and Early Holocene (14,600–8300 years ago) were represented by various environmental and climate changes and a transition from a cold to a warm climate, with subsequent changes in flora and fauna. Using Lake Āraisi as a case study (Latvia, northeastern Europe), local to regional vegetation was reconstructed by analyzing plant macroremains and pollen from the lake sediment profile. Here, we present the first dung fungus spore-based qualitative reconstruction of l…