0000000000225635

AUTHOR

J. Prat

showing 4 related works from this author

Molecular Epidemiology of Caliciviruses Causing Outbreaks and Sporadic Cases of Acute Gastroenteritis in Spain

2002

ABSTRACT The molecular epidemiology of human caliciviruses (HuCVs) causing sporadic cases and outbreaks of acute gastroenteritis around eastern Spain (Catalonia and the Valencian Community) was studied by reverse transcription-PCR (RT-PCR) and by sequencing part of the RNA polymerase gene in open reading frame 1. HuCVs were detected in 44 of 310 stool specimens (14.19%) negative for other enteric pathogens obtained from children with acute gastroenteritis. Norwalk-like viruses (NLVs) were the most common cause of the gastroenteritis outbreaks investigated here. They were detected in 14 out of 25 (56%) outbreaks with an identified pathogen. Genotypes producing both sporadic cases and outbrea…

Microbiology (medical)virusesMolecular Sequence DataBiologySapovirusVirusDisease Outbreakslaw.inventionlawVirologyGenotypeHumansPathogenGenePhylogenyPolymerase chain reactionFecesCaliciviridae InfectionsMolecular EpidemiologyMolecular epidemiologyReverse Transcriptase Polymerase Chain ReactionNorovirusOutbreakDNA-Directed RNA PolymerasesSequence Analysis DNAVirologyGastroenteritisSpainChild PreschoolAcute DiseaseCaliciviridaeJournal of Clinical Microbiology
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Search for supersymmetric particles using acoplanar charged-particle pairs from Z0 decays

1990

We have performed a search for supersymmetric particles using acoplanar pairs of oppositely-charged particles in decays of the Z0. In 0.53 pb−1 of integrated luminosity near the Z0 peak, we observe two events where approximately four are expected from background, allowing limits to be extended on combined photino and slepton masses, and also on combined photino and chargino masses.

PhysicsNuclear and High Energy PhysicsParticle physicsLuminosity (scattering theory)Electron–positron annihilationHigh Energy Physics::PhenomenologyElementary particleSupersymmetryCharged particleNuclear physicsCharginoHigh Energy Physics::ExperimentPhotinoParticle Physics - ExperimentBosonPhysics Letters B
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ALEPH: a Detector for Electron-Positron Annihilations at LEP

1990

Process-centred Software Engineering Environments (PSEE) are the most recent generation of environments supporting software development activities. Most of PSEE are based on mechanisms promoting enforcement and automation of process activities. In this kind of mechanisms the process models are prescribed in a detailed and complete way. But the experience shows that supporting processes is more concerned with the flexibility of guidance offered during the process performance than with enforcement of a collection of predefined process models. In this paper, we present a solution to support strategic processes in a PSEE by providing a flexible guidance during process enactment.

PhysicsFlexibility (engineering)Nuclear and High Energy PhysicsAlephhigh-energy physicsProcess modelingProcess (engineering)business.industrySoftware developmentLEPAutomationparticle detectorsData acquisitionDetectors and Experimental TechniquesLEP; particle detectors; high-energy physicsSoftware engineeringbusinessEnforcementInstrumentationparticle detector
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Evaluation of reverse transcription and polymerase chain reaction (RT/PCR) for the detection of rotaviruses: applications of the assay.

1996

Summary Our aim was to evaluate the reverse transcription and polymerase chain reaction (RT/PCR) technique for the detection of rotavirus shedding by infected children as a routine diagnostic procedure, in comparison to the enzyme-linked immunosorbent assay (ELISA), electron microscopy (EM) and polyacrylamide gel etectrophoresis (PAGE) of rotavirus double stranded RNA. Two-hundred and twenty stool specimens were collected from infants and young children with diarrhoea, and 10–20% faecal suspensions were made. Several methods of rotavirus dsRNA extraction were assayed. Electrophoretic analysis of viral RNA was carried out on 10% polyacrylamide gols followed by silver staining. RT/PCR was per…

RotavirusRT/PCRTranscription GeneticImmunologyReoviridaeEnzyme-Linked Immunosorbent AssayMicroscopic électroniquemedicine.disease_causeSensibilité.Polymerase Chain ReactionSensitivity and SpecificityRotavirus InfectionsArticlelaw.inventionFecesfluids and secretionsSensitivitylawVirologyRotavirusViral analysismedicineElectron microscopyHumansTypingChildRotavirus RT/PCRPolymerase chain reactionbiologyRNAInfantbiology.organism_classificationVirologyMolecular biologyReverse transcriptaseVirus SheddingPAGEMicroscopy ElectronReal-time polymerase chain reactionEvaluation Studies as TopicAnalyse viraleRNA ViralElectrophoresis Polyacrylamide GelELISARNA extractionResearch in virology
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