0000000000227352
AUTHOR
Ari Helenius
Characterizing a dc-SQUID and a Mo/TiW/Cu -trilayer transition-edge sensor array
Tutkielmassa määritettiin uuden suprajohtavan kvantti-interferenssilaitteen ja 256 pikseliä sisältävän sirun transitioreunailmaisimien ominaisuuksia. Komponentit vaativat erittäin matalia lämpötiloja ja suojauksen suurilta magneettikentiltä muuttuakseen suprajohtaviksi. Tärkeimmät mitatut ominaisuudet olivat suprajohtavan kvantti-interferenssilaitteen signaalin vahvistuskerroin sekä kohina ja transitioreunailmaisimen transitiolämpötila, normaalitilan vastus, virta-jännite-käyttäytyminen, sekä kohina. Motivaationa tutkimukselle oli etsiä käytetyn mittalaite kokonaisuuden herkkyyden rajat sekä pyrkiä sovittamaan teoreettista mallia transitioreunailmaisimen kohinan ymmärtämiseksi. Korkean taaj…
Single-cell analysis of population context advances RNAi screening at multiple levels
Isogenic cells in culture show strong variability, which arises from dynamic adaptations to the microenvironment of individual cells. Here we study the influence of the cell population context, which determines a single cell's microenvironment, in image‐based RNAi screens. We developed a comprehensive computational approach that employs Bayesian and multivariate methods at the single‐cell level. We applied these methods to 45 RNA interference screens of various sizes, including 7 druggable genome and 2 genome‐wide screens, analysing 17 different mammalian virus infections and four related cell physiological processes. Analysing cell‐based screens at this depth reveals widespread RNAi‐induce…
Echovirus 1 Endocytosis into Caveosomes Requires Lipid Rafts, Dynamin II, and Signaling EventsV⃞
Binding of echovirus 1 (EV1, a nonenveloped RNA virus) to the α2β1 integrin on the cell surface is followed by endocytic internalization of the virus together with the receptor. Here, video-enhanced live microscopy revealed the rapid uptake of fluorescently labeled EV1 into mobile, intracellular structures, positive for green fluorescent protein-tagged caveolin-1. Partial colocalization of EV1 with SV40 (SV40) and cholera toxin, known to traffic via caveosomes, demonstrated that the vesicles were caveosomes. The initiation of EV1 infection was dependent on dynamin II, cholesterol, and protein phosphorylation events. Brefeldin A, a drug that prevents SV40 transport, blocked the EV1 infection…