0000000000229650
AUTHOR
Antra Bormane
Ixodes ricinus L. un Ixodes persulcatus P.Sch. (Acari: Ixodidae) izplatība, to pārnēsāto infekcijas slimību nozīme un molekulārā epidemioloģija Latvijā
Elektroniskā versija nesatur pielikumus
Identification of Three Clinically Relevant Borrelia burgdorferi Sensu Lato Genospecies by PCR-Restriction Fragment Length Polymorphism Analysis of 16S-23S Ribosomal DNA Spacer Amplicons
ABSTRACT We report the results of a study of the prevalences of three clinically relevant Borrelia burgdorferi sensu lato genospecies ( Borrelia burgdorferi sensu stricto, Borrelia afzelii , and Borrelia garinii ) in 1,040 questing Ixodes ticks from all regions of Latvia, where Lyme borreliosis is endemic. The prevalences of Borrelia in Ixodes ricinus and Ixodes persulcatus were 22.6 and 27.9%, respectively. Molecular typing of B. burgdorferi from infected ticks was performed by restriction fragment length polymorphism (RFLP) analysis of PCR-amplified fragments of the 16S-23S ( rrs-rrlA ) rRNA intergenic spacer by using species-specific primers and subsequent sequencing. The dominant Borrel…
Vectors of tick-borne diseases and epidemiological situation in Latvia in 1993-2002.
During the period of 1993-2002 an increase and the remarkable changes in the incidence of tick-borne encephalitis (TBE) and Lyme borreliosis (LB) as well as annual activities of vector species were noticed. The highest increase of TBE morbidity in Latvia has been observed in 1994 and 1995, and less expressed also in 1998 which was followed by a significant decrease during subsequent years. Whereas the highest peak of LB morbidity has been noticed in 1998 with only a minor decrease during subsequent years. Two epidemiologically significant Ixodes tick species are common in Latvia. Ixodes ricinus L. spread in the western and central part of Latvia; but rarely and in small numbers also in the …