0000000000234077
AUTHOR
Günter Stein
Temporary warm ischaemia, 5/6 nephrectomy and single uranylnitrate administration — comparison of three models intended to cause renal fibrosis in rats
In patients the progression of pathologic renal processes after the treatment of primary disease is a problem of increasing importance and therapeutic strategies are insufficient till now. The aim of this paper was to search for rat models of interstitial fibrosis as a basis for testing therapeutic strategies to prevent end-stage renal failure. Experiments were done on adult female Wistar rats (Han:Wist) to investigate long-term consequences of temporary warm ischaemia, 5/6 nephrectomy (5/6 NX) and single uranyl nitrate (UN) administration (0.3 or 0.5 mg/ 100 g body wt. intraperitoneally). Observation time was 20 weeks after injury in each group. Creatinine clearance, urinary protein excret…
Expression of LFA-1 (CD11a/CD18) and ICAM-1 (CD54) in an animal model of renal interstitial fibrosis induced by unilateral ureteral obstruction
Unilateral ureteral obstruction (UUO) has been used as an experimental model to induce tubulointerstitial damage and interstitial fibrosis. UUO is characterized by cellular proliferation, accumulation of inflammatory cells, and subsequent replacement of renal parenchyma by fibrous tissue. The influx of inflammatory cells into the renal interstitium is mediated by adhesion molecules. In this study, the development of fibrosis in the UUO model of the rat was examined and its relation to the time course of LFA-1 and ICAM-1 expression was assessed by immunohistochemistry. An increase in interstitial connective tissue was detected on day 10 after UUO, with a maximum on day 35. After unilateral u…
Kinetische untersuchungen zur reaktionsfähighkeit von chlormethylgruppen in methylendiphenolen
The chloromethyl derivatives of 16 differently substituted methylenediphenols were synthesized for the first time. To compare their reactivity, the solvolysis in methanol and the animolysis with an excess of p-nitroaniline in dimethyl sulfoxide were studied kinetically at 25°C. The rate constants for the methanolysis do not differ very much, beside of the fact that para-chloromethyl derivatives react about 100 times faster than ortho-chloromethyl derivatives. However, for the aminolysis a large acceleration up to 103 was found for compounds having both hydroxyl groups in ortho-position to the methylene bridge. Obviously, the cleavage of the OH bond, which occurs in the rate determining step…