0000000000236031

AUTHOR

Esther Bataller

Novel Probiotic Bifidobacterium bifidum CECT 7366 Strain Active against the Pathogenic Bacterium Helicobacter pylori▿

ABSTRACT Helicobacter pylori is considered one of the major risk factors underlying the development of gastritis and gastric and duodenal ulcers. Moreover, 50% of the population carries this bacterium, and consequently, when it is detected, eradication of H. pylori is strongly recommended. Regarding the use of probiotics as functional agents, several studies have shown that there is a direct relationship between the addition of certain probiotic bacteria and in vitro inhibition of H. pylori ; however, in vivo studies showing bifidobacterial activity against H. pylori remain scarce. In this study, a Bifidobacterium bifidum strain which proved active in vitro against H. pylori has been isolat…

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Novel Probiotic Bifidobacterium longum subsp. infantis CECT 7210 Strain Active against Rotavirus Infections

ABSTRACT Rotavirus is the leading cause of severe acute gastroenteritis among children worldwide. It is well known that breast-feeding and vaccination afford infants protection. Since breast-feeding has drastically decreased in developed countries, efforts have been focused on the potential use of probiotics as preventive agents. In this study, a novel Bifidobacterium longum subsp. infantis strain was isolated from infant feces and selected, based on its capacity to inhibit in vitro rotavirus Wa replication (up to 36.05% infectious foci reduction) and also to protect cells from virus infection (up to 48.50% infectious foci reduction) in both MA-104 and HT-29 cell lines. Furthermore, studies…

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Bacteria from acidic to strongly alkaline insect midguts: Potential sources of extreme cellulolytic enzymes

Abstract Bacterial isolates from the European Corn Borer (ECB) Ostrinia nubilalis and the coleopteran Colorado Potato Beetle (CPB) Leptinotarsa decemlineata midguts were identified and characterized. Twenty-four colonies, selected on solid media with cellulose as the sole carbon source and pH values ranging from 5 to 12, were identified through 16S sequencing as members of the genera Acinetobacter , Comamonas , Klebsiella , Microbacterium , Micrococcus and Sphingobacterium . A complete enzymatic characterization revealed widespread – albeit moderate – cellulase properties in all but one isolate and high xylanase activity in the four CPB isolates. Different enzymatic patterns in terms of opt…

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