First stages of microsporere programming to embryogenesis through anther culture in Prunus armeniaca L.
6 páginas, 3 figuras, 2 tablas -- PAGS nros. 152-157
FIRST STAGES OF MICROSPORE REPROGRAMMING TO EMBRYOGENESIS THROUGH ISOLATED MICROSPORE CULTURE IN LOQUAT
The current experiments were undertaken to develop a method for regenerating doubled haploid (DH) plants of Eriobotrya japonica (Thunb.), Rosaceae, subfamily Maloideae through isolated microspore culture of nine loquat cultivars. Protocols were developed for isolation and culture of loquat microspores. Isolated microspores in vitro cultured started dividing and developing multicellular, globular and irregular structures. After 4 weeks of culture, the responding microspores produced yellowish/white callus. The embryogenic response of microspores was highly dependent on the cultivar and on the medium composition.
Induction of pollen embryogenesis in seven cultivars of Prunus armeniaca l.
6 páginas -- PAGS nros. 273-278
Development of multicellular pollen of Eriobotrya japonica Lindl. through anther culture
8 pages, 5 figures.-- Printed version published Dec 2006.
Early in vitro development of isolated microspores of four Sicilian olive cultivars.
Nowadays there is a great interest in the advancement of the studies on gametic embryogenesis in olive, as a method for obtaining homozygous material, due to the enormous effort and relevance in its genome sequencing and gene mapping. In this study, in vitro isolated microspore culture of four Sicilian olive cultivars (‘Biancolilla napoletana’, ‘Nocellara del Belice’, ‘Tonda Iblea’ and ‘Verdello’) has been carried out, investigating the influence of two polyamines (putrescine and spermidine) and of two medium compositions on pollen embryogenesis induction. During in vitro development, different structural features have been observed: uninucleated microspores, with no development, binucleate…
Autophagy
In 2008, we published the first set of guidelines for standardizing research in autophagy. Since then, this topic has received increasing attention, and many scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Thus, it is important to formulate on a regular basis updated guidelines for monitoring autophagy in different organisms. Despite numerous reviews, there continues to be confusion regarding acceptable methods to evaluate autophagy, especially in multicellular eukaryotes. Here, we present a set of guidelines for investigators to select and interpret methods to examine autophagy and related processes, and for reviewers to provide…
Early embryo achievement through isolated microspore culture in citrus clementina hort. Ex tan., cvs. 'Monreal Rosso' and 'nules'
10 p.-5 fig.-3 tab.