0000000000237328

AUTHOR

Angel Domínguez

showing 5 related works from this author

CandidaDB: a genome database for Candida albicans pathogenomics.

2004

CandidaDB is accessible at http://genolist.pasteur.fr/CandidaDB.; International audience; CandidaDB is a database dedicated to the genome of the most prevalent systemic fungal pathogen of humans, Candida albicans. CandidaDB is based on an annotation of the Stanford Genome Technology Center C.albicans genome sequence data by the European Galar Fungail Consortium. CandidaDB Release 2.0 (June 2004) contains information pertaining to Assembly 19 of the genome of C.albicans strain SC5314. The current release contains 6244 annotated entries corresponding to 130 tRNA genes and 5917 protein-coding genes. For these, it provides tentative functional assignments along with numerous pre-run analyses th…

Protein familyGenomicsComputational biologyBiologyGenomeANNOTATIONFUNCTIONNAL ASSIGNEMENTFungal ProteinsUser-Computer Interface03 medical and health sciencesAnnotationPathogenomicsCandida albicansDatabases GeneticGeneticsGENOME DATABASE[SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry Molecular Biology/Biochemistry [q-bio.BM]GeneComputingMilieux_MISCELLANEOUS030304 developmental biologyWhole genome sequencingGeneticsInternet0303 health sciencesFungal protein030306 microbiologygénomeEUROPEAN CONSORTIUMGENOME SEQUENCEGenomicsArticlesGENOME DATABASE;CANDIDA DB;ANNOTATION;GENOME SEQUENCE;GENE FUNCTION;EUROPEAN CONSORTIUM;FUNCTIONNAL ASSIGNEMENTGENE FUNCTIONCANDIDA DB[SDV.MP]Life Sciences [q-bio]/Microbiology and ParasitologyGenome FungalNucleic Acids Research
researchProduct

ABG1 , a Novel and Essential Candida albicans Gene Encoding a Vacuolar Protein Involved in Cytokinesis and Hyphal Branching

2005

ABSTRACT Immunoscreening of a Candida albicans expression library resulted in the isolation of a novel gene encoding a 32.9-kDa polypeptide (288 amino acids), with 27.7% homology to the product of Saccharomyces cerevisiae YGR106c, a putative vacuolar protein. Heterozygous mutants in this gene displayed an a ltered b udding g rowth pattern, characterized by the formation of chains of buds, decreasingly in size towards the apex, without separation of the daughter buds. Consequently, this gene was designated ABG1 . A conditional mutant for ABG1 with the remaining allele under the control of the MET3 promoter did not grow in the presence of methionine and cysteine, demonstrating that ABG1 was e…

Blotting WesternGreen Fluorescent ProteinsSaccharomyces cerevisiaeMutantHyphaeVacuoleVacuole inheritanceMicrobiologyFungal ProteinsGene Expression Regulation FungalCandida albicansCloning MolecularCandida albicansMolecular BiologyGeneCytokinesisFungal proteinGenes EssentialBase SequencebiologyArticlesGeneral Medicinebiology.organism_classificationBiochemistryVacuolesElectrophoresis Polyacrylamide GelGenome FungalCytokinesisSubcellular FractionsEukaryotic Cell
researchProduct

Null mutants of Candida albicans for cell-wall-related genes form fragile biofilms that display an almost identical extracellular matrix proteome.

2016

By two-dimensional gel electrophoresis (2-DE) and mass spectrometry, we have characterized the polypeptide species present in extracts obtained by 60% ethanol treatment of whole mature (48 h) biofilms formed by a reference strain (CAI4- URA3 ) and four Candida albicans null mutants for cell-wall-related genes ( ALG5, CSA1, MNN9 and PGA10) . Null mutants form fragile biofilms that appeared partially split and weakly attached to the substratum contrary to those produced by the reference strain. An almost identical, electrophoretic profile consisting of about 276 spots was visualized in all extracts examined. Proteomic analysis led to the identification of 131 polypeptides, corresponding to 86…

0301 basic medicineSignal peptideProteomeMutantGenes FungalBiologyApplied Microbiology and BiotechnologyMicrobiologyMass SpectrometryCell wallFungal Proteins03 medical and health sciencesCell WallCandida albicansElectrophoresis Gel Two-DimensionalCandida albicansGel electrophoresisMolecular massBiofilmGeneral Medicinebiology.organism_classificationMolecular biologyExtracellular Matrix030104 developmental biologyBiofilmsProteomeGene DeletionFEMS yeast research
researchProduct

Regulation of chitin synthase activity inSaccharomyces cerevisiae: Effect of the inhibition of cell division and of synthesis of RNA and protein

1980

The effect of pronase and trypsin on the activation or deactivation (degradation?) of chitin synthase ofSaccharomyces cerevisiae occurs faster in membranous preparations than in toluene-treated cells. When the temperature is raised, the former preparation is deactivated earlier than the latter one. The activity found in growing cells is not modified after inhibition of protein synthesis by cycloheximide or amino acid starvation or by the inhibition of RNA synthesis. It was possible to activate the chitin synthase ofS. cerevisiae cdc 25 grown at 23°C by means of pronase, whereas trypsin had no effect. After the cells were grown at 37°C, chitin synthase could not be activated either with tryp…

chemistry.chemical_classificationCell divisionRNAGeneral MedicinePronaseChitin synthaseCycloheximideBiologyTrypsinApplied Microbiology and BiotechnologyMicrobiologyAmino acidchemistry.chemical_compoundchemistryBiochemistrymedicinebiology.proteinProtein biosynthesismedicine.drugCurrent Microbiology
researchProduct

Mutants of Saccharomyces cerevisiae cell division cycle defective in cytokinesis. Biosynthesis of the cell wall and morphology

1982

The four temperature-sensitive mutants of Saccharomyces cerevisiae in the cell division cycle defective in cytokinesis (cdc, 3, 10, 11 and 12), have been analyzed with respect to the biosynthesis of the cell wall polymers. After 3 hours of incubation at the non-permissive temperature (37 degrees C) these strains stop growing. The synthesis of glucan, mannan and chitin (wall polymers) level off in a similar time, but glucan, mannan and chitin synthases remained active for at least 4 hours. If the mutants are analyzed by transmission and scanning electron microscopy different pictures emerge. Two of the mutants cdc 10 and cdc 12, after 3 hours of incubation at 37 degrees C present apparently …

MutantSaccharomyces cerevisiaeChitinSaccharomyces cerevisiaemacromolecular substancesSeptinMicrobiologyMannansCell wallchemistry.chemical_compoundChitinCell WallTelophaseGlucansMolecular BiologyMannanGlucanchemistry.chemical_classificationbiologyCell MembraneGeneral Medicinebiology.organism_classificationcarbohydrates (lipids)chemistryBiochemistryMutationCell DivisionCytokinesisAntonie van Leeuwenhoek
researchProduct