Study of the role of the CDC48 chaperone protein in plant immunity

The chaperone protein CDC48 (Cell division cycle 48) is a major regulator of the quality control of proteins and is involved in various cellular processes in animals and yeast. In contrast, the role of CDC48 in plants is poorly known. In the present work, we investigated the function of CDC48 in plant immunity thanks to the cryptogein/tobacco biological model, cryptogein being produced by the oomycete phytophthora cryptogea.Three strategies were carried out. First, the dynamic of accumulation CDC48 together with intracellular events inherent to the immune response were analyzed in both wild-type and CDC48 overexpressing tobacco cells (CDC48-TAP line). Second, a list if CDC48 partners was es…

New insights about the role of the chaperon-like protein Cdc48, a target for nitric oxide in plant immunity

Functional characterization of the chaperon-like protein Cdc48 in cryptogein-induced immune response in tobacco. Communication orale et poster

International audience; Cdc48, a molecular chaperone conserved in diff erent kingdoms, is a member of the AAA+ family contributing to numerous processes in mammalian including proteins quality control and degradation, vesicular traffi cking, autophagy and immunity. The functions of Cdc48 plant orthologues are less understood. We previously reported that Cdc48 is regulated by S-nitrosylation in tobacco cells undergoing an immune response triggered by cryptogein, an elicitin produced by the oomycete Phytophthora cryptogea. Here, we investigated the function of NtCdc48 in cryptogein signaling and induced hypersensitive-like cell death. NtCdc48 was found to accumulate in elicited cells both at …

Study of the chaperone protein CDC48 and its involvement in plant immunity

CDC48 is a conserved chaperone protein belonging to the AAA+ ATPase family (ATPase associated with various activities). This protein uses binding and hydrolysis of ATP to generate forces to affect the transformation of polypeptide substrate in numerous cellular processes. Studies on mammalian CDC48 orthologue revealed that it recognizes ubiquitylated polypeptides, directly or via partners, leading to substrate degradation or recycling. In plants, functions of CDC48 is less understood. The aim of my thesis, is to decipher the role of CDC48 in plant defense response context. First, I have to characterize NtCDC48 in tobacco (Nicotiana tabacum) suspension cells elicited by cryptogein, an elicit…