0000000000240426
AUTHOR
Piotr Zdańkowski
showing 4 related works from this author
DarkFocus: numerical autofocusing in digital in-line holographic microscopy using variance of computational dark-field gradient
2020
Abstract We report on a novel computational technique for automatic numerical refocusing in digital in-line holographic microscopy. It is based on the adaptive filtering of the recorded on-axis hologram to eliminate its background term and extract interference intensity-component connected with light scattered on the sample (interference fringes). Numerical propagation of such filtered hologram yields the computationally generated dark-field imaging coming from the amplitude part of the complex field. We propose a simple measure in the form of the variance of the dark-field gradient, which attains its maximum value in the focal planes for all types of objects (phase, amplitude and mixed pha…
Versatile optimization-based speed-up method for autofocusing in digital holographic microscopy
2021
We propose a speed-up method for the in-focus plane detection in digital holographic microscopy that can be applied to a broad class of autofocusing algorithms that involve repetitive propagation of an object wave to various axial locations to decide the in-focus position. The classical autofocusing algorithms apply a uniform search strategy, i.e., they probe multiple, uniformly distributed axial locations, which leads to heavy computational overhead. Our method substantially reduces the computational load, without sacrificing the accuracy, by skillfully selecting the next location to investigate, which results in a decreased total number of probed propagation distances. This is achieved by…
Single-shot two-frame π-shifted spatially multiplexed interference phase microscopy
2019
Single-shot, two-frame, π-shifted spatially multiplexed interference microscopy (π-SMIM) is presented as an improvement to previous SMIM implementations, introducing a versatile, robust, fast, and accurate method for cumbersome, noisy, and low-contrast phase object analysis. The proposed π-SMIM equips a commercially available nonholographic microscope with a high-speed (video frame rate) enhanced quantitative phase imaging (QPI) capability by properly placing a beam-splitter in the microscope embodiment to simultaneously (in a single shot) record two holograms mutually phase shifted by π radians at the expense of reducing the field of view. Upon subsequent subtractive superimposition of hol…
Upgrading a brightfield optical microscope into a robust numerically advanced interference-based phase imager
2019
The approach to convert a brightfield microscope into an interference-based versatile quantitative phase imaging unit is presented. It employs partially coherent illumination and diffraction grating. Enhanced interferogram bio-phase retrieval is performed by two-shot numerically-robust Hilbert-Huang method.