0000000000241322

AUTHOR

Worawee Waiyawuth

showing 2 related works from this author

Tandem repeat structure of the duplicated Y-chromosomal STR locus DYS385 and frequency studies in the German and three Asian populations

1998

The Y-chromosomal short tandem repeat (STR) locus DYS385 can be typed using PCR amplification and separation of the resulting polymorphic fragments by non-denaturing high resolution polyacrylamide gel electrophoresis followed by silver staining. The PCR primers amplify a duplicated repeat sequence on the Y chromosome revealing a two-band pattern in male individuals. To determine the internal repeat structure as a basis for a consensus nomenclature, DNA sequence analysis was carried out after subcloning of PCR-amplified fragments revealing the uniform 4-bp repeat structure 'GAAA'. The shortest allele observed consisted of 10 repeat units thus providing the basis for the designation 'allele 1…

MaleGenotypePopulationLocus (genetics)BiologyPolymerase Chain ReactionPathology and Forensic MedicineGene FrequencyTandem repeatGermanyGenotypeHumanseducationAllele frequencyAsia SoutheasternGeneticseducation.field_of_studyPolymorphism GeneticBase SequenceGenetic VariationSequence Analysis DNAGenotype frequencyGenetics PopulationSTR analysisTandem Repeat SequencesMicrosatelliteElectrophoresis Polyacrylamide GelLawForensic Science International
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Genetic analysis of the short tandem repeat system D12S391 in the German and three Asian populations

1998

Abstract Genomic DNA samples from 222 individuals from Southern China, 154 individuals from Thailand, 100 individuals from Japan as well as from 124 German individuals were analysed for the short tandem repeat (STR) locus D12S391. Typing was carried out by polymerase chain reaction (PCR) amplification and subsequent polyacryramide gel electrophoresis and silver staining. In total, 12 alleles could be distinguished in two of the populations. Among Chinese, allele 19 is the most common with a frequency of 0.225, and among Germans, allele 18 with a frequency of 0.186. In the Thai population only 11 alleles could be distinguished and allele 19 is the most common with a frequency of 0.198. In Ja…

MaleAsiaGenotypeLocus (genetics)BiologyPolymerase Chain ReactionGenetic analysisPathology and Forensic MedicineGermanyGenotypeHumansFluorometryAlleleChildAllele frequencyAllelesDNA PrimersRepetitive Sequences Nucleic AcidGeneticsDNAGenotype frequencyGenetics PopulationGenetic markerMicrosatelliteElectrophoresis Polyacrylamide GelFemaleLawForensic Science International
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