0000000000241843
AUTHOR
Z. Kalnina
Molecular characterisation and expression analysis of SEREX-defined antigen NUCB2 in gastric epithelium, gastritis and gastric cancer.
NUCB2 is an EF-hand Ca2+ binding protein that has been implicated in various physiological processes like calcium homeostasis, hypothalamic regulation of feeding and TNF receptor shedding. In our previous study we identified NUCB2 as a potential tumour antigen eliciting autoantibody responses in 5.4% of gastric cancer patients but not in the healthy individuals.The current study aimed to elucidate the molecular mechanism underlying NUCB2 immunogenicity and to gain an insight into the physiological functions of NUCB2 in the stomach. mRNA expression analysis demonstrated that NUCB2 is ubiquitously expressed in normal tissues, including lymphoid tissues, and downregulated in gastric tumours wh…
Survey of autoantibody responses against tumor-associated antigens in thyroid cancer
Background Autoantibodies against tumor-associated antigens (TAAs) have been shown to serve as highly specific serological biomarkers for the diagnosis of various solid cancers. Although the autoimmunity against thyroid tissue specific antigens has been studied extensively, so far, the autoantibody responses against common TAAs such as cancer-testis antigens (CTAs), mutated or differentiation antigens have not been comprehensively analyzed in patients with thyroid cancer. Objective The current study aims to characterize the frequency of autoantibody responses against common TAAs in patients with thyroid cancer and benign thyroid nodules. Methods A phage-displayed antigen microarray comprisi…
PO-224 Fourier transform infrared spectroscopy-based detection of hypoxia-induced biomolecular changes in different cancer cell lines
Introduction Our previous studies have shown that under hypoxic stress human colorectal and breast cancer cells produce higher amounts of lipids compared to that under normoxic environment. The aim of this study was to estimate hypoxia induced cell metabolic response via the biochemical composition in prostate and lung cancer cells by using Fourier transform infrared spectroscopy (FTIR). Material and methods Lung cancer cell lines NCI-H69, COR-L23 and prostate cancer cell line PC3, and primary prostate cancer culture NFI-56 were seed in serum-free medium in density 1 × 106 per well in triplicates in 6-well plates and incubated for 48 in normoxic and hypoxic (5% CO2, 1% O2 and 94% N2) condit…